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. 2014 Jun 2:3:280.
doi: 10.1186/2193-1801-3-280. eCollection 2014.

Microbiota from Litopenaeus vannamei: digestive tract microbial community of Pacific white shrimp (Litopenaeus vannamei)

Jaqueline Tuyub Tzuc  1 , Diana Rendíz Escalante  2 , Rafael Rojas Herrera  2 , Gabriela Gaxiola Cortés  1 , Maria Leticia Arena Ortiz  1
Affiliations

Microbiota from Litopenaeus vannamei: digestive tract microbial community of Pacific white shrimp (Litopenaeus vannamei)

Jaqueline Tuyub Tzuc et al. Springerplus. .

Abstract

Bacteria capable of producing different extracellular enzymes of potential relevance in digestive processes were isolated from the stomach, hepatopancreas and intestine of Pacific white shrimp Litopenaeus vannamei. A total of 64 strains with proteolytic activity were isolated and grouped into 16 clusters based on morphological characteristics: 4 groups were isolated from the intestine; 5 from the hepatopancreas; and 7 from the stomach. Molecular methods (16S rRNA gene amplification and sequencing) and phenotypic criteria (Gram stain, catalase and oxidase tests, cell and colony morphology) were used to identify strains, which corresponded to Pseudoalteromonas and Vibrio genera. These genera are reported to form part of the digestive tract microbial community in shrimp. Both genera were isolated from all three tested tissues. One member of each morphologic group was selected for analysis of the presence of amylases, lipases/esterases and chitinases. Most of the strains had all the tested enzymes, indicating that the L. vannamei digestive tract microbiotic flora includes groups which have the potential to contribute to the degradation of dietary components.

Keywords: 16S; Digestion; Enzymatic activity; Microbiota; Shrimp.

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Figures

Figure 1
Figure 1
Phylogenetic tree based on RNAr 16S gene sequences (Ratchet method, Length: 317, consistency index: 70%, retention index: 95%). We show the phylogenetic relation of the studied strains with other groups. Micrococcus luteus AJ536198 was used as an external group.
Figure 2
Figure 2
Phylogenetic tree based on RNAr 16S gene sequences (Ratchet method, Length: 307, consistency index: 60%, retention index: 91%). We show the phylogenetic relation of the studied strains with other groups. Micrococcus luteus AJ536198 was used as an external group.
See this image and copyright information in PMC

References

    1. Aghajari N, Feller G, Gerday C, Haser R. Crystal structures of the psychrophilic a-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor. Protein Sci. 1998;7:564–572. doi: 10.1002/pro.5560070304. - DOI - PMC - PubMed
    1. Akiyama D, Dominy W, Lawrence A. Penaeid shrimp nutrition for the commercial feed industry. In: Akiyama D, Tan R, editors. Proceedings of the aquaculture feed processing and nutrition workshop. Singapore: American Soybean Association; 1991. pp. 80–98.
    1. Atlas RM. Handbook of Media for Environmental Microbiology. 2. 2005. p. 664.
    1. Avilés-Gómez L. Masters Thesis. 2011. Estudio de la microbiota del sistema digestivo en el camarón blanco Litopenaeus vannamei; p. 104.
    1. Balcázar J, Rojas-Luna T, Cunningham D. Effect of the addition of four potential probiotics strains on the survival of Pacific white shrimp (Litopenaeus vannamei) following immersion challenge with Vibrio parahaemolyticus. J Invertebr Pathol. 2007;96:147–150. doi: 10.1016/j.jip.2007.04.008. - DOI - PubMed