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. 2014 Jun 24:20:1056-66.
doi: 10.12659/msm.890638.

The in vitro biological properties of Mg-Zn-Sr alloy and superiority for preparation of biodegradable intestinal anastomosis rings

Ling Liu  1 , Nianfeng Li  1 , Ting Lei  2 , Kaimo Li  3 , Yangde Zhang  3
Affiliations

The in vitro biological properties of Mg-Zn-Sr alloy and superiority for preparation of biodegradable intestinal anastomosis rings

Ling Liu et al. Med Sci Monit. .

Abstract

Background: Magnesium (Mg) alloy is a metal-based biodegradable material that has received increasing attention in the field of clinical surgery, but it is currently seldom used in intestinal anastomosis. This study was conducted to comprehensively assess a ternary magnesium (Mg)-zinc (Zn)-strontium (Sr) alloy's biological superiorities as a preparation material for intestinal anastomosis ring.

Material and methods: Mouse L-929 fibroblasts were cultured with Mg-Zn-Sr alloy extract and compared with both positive (0.64% phenol) and negative (original broth culture) controls. The cell morphology of different groups was examined using microscopy, and a cytotoxicity assessment was performed. Fresh anticoagulated human blood was mixed with Mg-Zn-Sr alloy extract and compared with both positive (distilled water) and negative (normal saline) controls. The absorbance of each sample at 570 nm was used to calculate the Mg-Zn-Sr alloy hemolysis ratio in order to test the Mg alloy's blood compatibility. Bacterial cultures of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were added to Mg-Zn-Sr alloy block samples and compared with positive (Ceftazidime), negative (316LSS stainless steel), and blank controls. The broth cultures were sampled to compare their bacterial colony counts so as to evaluate the antibacterial properties of the Mg-Zn-Sr alloy. The Mg-Zn-Sr alloy was surface-coated with a layer of poly(lactic-co-glycolic acid) carrying everolimus. The surface morphology and degradability of the coating were examined so as to demonstrate feasibility of coating, which can release the drug evenly.

Results: The experiments proved that Mg-Zn-Sr alloy has good biocompatible, antibacterial, and drug-loaded coating performances, which are lacking in existing intestinal anastomosis devices/materials.

Conclusions: The Mg-Zn-Sr alloy increases biocompatibility, and yields a safer and better therapeutic effect; therefore, it is a novel biomaterial that is feasible for use when preparing biodegradable intestinal anastomosis rings.

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Figures

Figure 1
Figure 1
Cell morphology in the 3 groups after 2,7-D incubation. Groups a, b, and c are the experimental group, positive control group, and negative control group, respectively. In the above chart, the cellular numbers of group a and c were more than in the corresponding group b, and the cellular number of group a was almost the same as group c.
Figure 2
Figure 2
The OD values of each group. The OD values for the positive control group are obviously lower than that of both the experimental group and negative control group.
Figure 3
Figure 3
OD values of each group at 570 nm. Fresh anticoagulated human blood was allowed to make contact with the Mg-Zn-Sr alloy extract (experimental group) and compared with the positive control group (0.64% phenol) and negative control group (normal saline). Six parallel samples were prepared for each group, and the absorbance of each sample was measured at 570 nm with an ultraviolet-visible spectrophotometer.
Figure 4
Figure 4
The 4 Escherichia coli culture groups (left to right: experimental group, blank control group with no additive, negative control group with 316L stainless steel, and positive control group with antibiotics). The magnesium alloy of the experimental group precipitated a corrosion reaction in the culture liquid, a large number of bubbles emerged on the surface of the liquid, and there are also obvious signs of corrosion on the surface of the magnesium alloy block, but no such phenomenon occurred in the control group.
Figure 5
Figure 5
The number of bacterial colonies on each plate. Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus with control, Mg-Zn-Sr alloy, 316LSS and ceftazidime treatment groups. Control, no additive; Mg-Zn-Sr, Mg-Zn-Sr alloy turnings; 316LSS, 316L stainless steel; ceftazidime, antibiotic. Data are presented as median CFU ml/L. Columns of the same group were not significantly different (P>0.05) at the given time point.
Figure 6
Figure 6
The coated Mg-Zn-Sr alloy’s Surface morphology by SEM. White points in the figure are some of the non-conductive dust and other substances on the surface.
Figure 7
Figure 7
PLGA coating’s weight loss-time coordinates.
Figure 8
Figure 8
Model of the intestinal anastomosis rings and the course of anastomotic operation with anastomosis ring model.
See this image and copyright information in PMC

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