Expression in Bacillus subtilis of the 51- and 42-kilodalton mosquitocidal toxin genes of Bacillus sphaericus
- PMID: 2495762
- PMCID: PMC184087
- DOI: 10.1128/aem.55.1.252-253.1989
Expression in Bacillus subtilis of the 51- and 42-kilodalton mosquitocidal toxin genes of Bacillus sphaericus
Abstract
A 3,080-base-pair KpnI-HindIII DNA fragment from Bacillus sphaericus 2362 coding for 51- and 42-kilodalton mosquitocidal proteins was cloned into Bacillus subtilis DB104 by using the vector pUB18. In B. subtilis these proteins were not detected during vegetative growth but were expressed during sporulation at levels comparable to those found in B. sphaericus.
Similar articles
-
The 42- and 51-kilodalton mosquitocidal proteins of Bacillus sphaericus 2362: construction of recombinants with enhanced expression and in vivo studies of processing and toxicity.J Bacteriol. 1990 May;172(5):2217-23. doi: 10.1128/jb.172.5.2217-2223.1990. J Bacteriol. 1990. PMID: 2110136 Free PMC article.
-
Gene dosage effect on the expression of the delta-endotoxin genes of Bacillus thuringiensis subsp. kurstaki in Bacillus subtilis and Bacillus megaterium.Gene. 1989 Jun 30;79(1):21-31. doi: 10.1016/0378-1119(89)90089-9. Gene. 1989. PMID: 2550328
-
Sequence analysis of the mosquitocidal toxin genes encoding 51.4- and 41.9-kilodalton proteins from Bacillus sphaericus 2362 and 2297.J Bacteriol. 1988 May;170(5):2045-50. doi: 10.1128/jb.170.5.2045-2050.1988. J Bacteriol. 1988. PMID: 3360740 Free PMC article.
-
Biochemical genetics of the bacterial insect-control agent Bacillus thuringiensis: basic principles and prospects for genetic engineering.Biotechnol Genet Eng Rev. 1984;2:341-63. doi: 10.1080/02648725.1984.10647804. Biotechnol Genet Eng Rev. 1984. PMID: 6443645 Review. No abstract available.
-
Sporulation of Bacillus subtilis.Curr Opin Microbiol. 2004 Dec;7(6):579-86. doi: 10.1016/j.mib.2004.10.001. Curr Opin Microbiol. 2004. PMID: 15556029 Review.
Cited by
-
Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes.Microbiol Rev. 1993 Dec;57(4):838-61. doi: 10.1128/mr.57.4.838-861.1993. Microbiol Rev. 1993. PMID: 7905597 Free PMC article. Review.
-
The 42- and 51-kilodalton mosquitocidal proteins of Bacillus sphaericus 2362: construction of recombinants with enhanced expression and in vivo studies of processing and toxicity.J Bacteriol. 1990 May;172(5):2217-23. doi: 10.1128/jb.172.5.2217-2223.1990. J Bacteriol. 1990. PMID: 2110136 Free PMC article.
-
Comparative genomics reveals Lysinibacillus sphaericus group comprises a novel species.BMC Genomics. 2016 Sep 5;17(1):709. doi: 10.1186/s12864-016-3056-9. BMC Genomics. 2016. PMID: 27595771 Free PMC article.
-
Bacillus sphaericus as a mosquito pathogen: properties of the organism and its toxins.Microbiol Rev. 1991 Sep;55(3):425-36. doi: 10.1128/mr.55.3.425-436.1991. Microbiol Rev. 1991. PMID: 1682792 Free PMC article. Review.
-
Regulation of mosquitocidal toxin synthesis in Bacillus sphaericus.Appl Microbiol Biotechnol. 1995 May-Jun;43(2):310-4. doi: 10.1007/BF00172830. Appl Microbiol Biotechnol. 1995. PMID: 7612250
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources