CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes
- PMID: 24957798
- PMCID: PMC4067614
- DOI: 10.1038/srep05228
CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes
Erratum in
- Sci Rep. 2014;4:6385
Abstract
Standard culture of human induced pluripotent stem cells (hiPSCs) requires basic Fibroblast Growth Factor (bFGF) to maintain the pluripotent state, whereas hiPSC more closely resemble epiblast stem cells than true naïve state ES which requires LIF to maintain pluripotency. Here we show that chemokine (C-C motif) ligand 2 (CCL2) enhances the expression of pluripotent marker genes through the phosphorylation of the signal transducer and activator of transcription 3 (STAT3) protein. Moreover, comparison of transcriptomes between hiPSCs cultured with CCL2 versus with bFGF, we found that CCL2 activates hypoxia related genes, suggesting that CCL2 enhanced pluripotency by inducing a hypoxic-like response.Further, we show that hiPSCs cultured with CCL2 can differentiate at a higher efficiency than culturing withjust bFGF and we show CCL2 can be used in feeder-free conditions [corrected]. Taken together, our finding indicates the novel functions of CCL2 in enhancing its pluripotency in hiPSCs.
Figures




References
-
- Takahashi K. & Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663–676, 10.1016/j.cell.2006.07.024 (2006). - PubMed
-
- Takahashi K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861–872, 10.1016/j.cell.2007.11.019 (2007). - PubMed
-
- Yu J. et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917–1920, 10.1126/science.1151526 (2007). - PubMed
-
- Nichols J. & Smith A. Naive and primed pluripotent states. Cell Stem Cell 4, 487–492, 10.1016/j.stem.2009.05.015 (2009). - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous