doi: 10.1128/jb.171.5.2653-2656.1989.
Stability of reiterated sequences in the Bacillus subtilis chromosome
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- PMID: 2496112
- PMCID: PMC209948
- DOI: 10.1128/jb.171.5.2653-2656.1989
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Stability of reiterated sequences in the Bacillus subtilis chromosome
J Bacteriol.
1989 May.
Abstract
The instability of reiterated sequences in the Bacillus subtilis chromosome that was previously reported (M. Young, J. Gen. Microbiol. 130:1613-1621, 1984) results from the presence of a truncated pC194 replication origin together with an intact replication protein A gene in the amplified DNA. Removal of the truncated pC194 replication origin or inactivation of replication protein A stabilizes reiterated sequences, whereas provision of replication protein A in trans destabilizes them. We suggest that residual activity of protein A at the truncated replication origin generates single-stranded DNA, which stimulates recombination between repeated sequences and thus destabilizes amplified structures.
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