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. 1989 May;171(5):2653-6.
doi: 10.1128/jb.171.5.2653-2656.1989.

Stability of reiterated sequences in the Bacillus subtilis chromosome

M Young  1 , S D Ehrlich
Affiliations

Stability of reiterated sequences in the Bacillus subtilis chromosome

M Young et al. J Bacteriol. 1989 May.

Abstract

The instability of reiterated sequences in the Bacillus subtilis chromosome that was previously reported (M. Young, J. Gen. Microbiol. 130:1613-1621, 1984) results from the presence of a truncated pC194 replication origin together with an intact replication protein A gene in the amplified DNA. Removal of the truncated pC194 replication origin or inactivation of replication protein A stabilizes reiterated sequences, whereas provision of replication protein A in trans destabilizes them. We suggest that residual activity of protein A at the truncated replication origin generates single-stranded DNA, which stimulates recombination between repeated sequences and thus destabilizes amplified structures.

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References

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