Indirect double sandwich ELISA for the specific and quantitative measurement of mouse IgM, IgA and IgG subclasses
- PMID: 2496168
- DOI: 10.1016/0022-1759(89)90388-8
Indirect double sandwich ELISA for the specific and quantitative measurement of mouse IgM, IgA and IgG subclasses
Abstract
We have developed sensitive enzyme-linked immunosorbent assays (ELISA) which measure mouse serum heavy chain immunoglobulin isotypes in nanograms per milliliter. In each case the specific isotypic Ig is sandwiched between an isotype-specific antibody used for coating and another isotype-specific antibody coupled to biotin for detection (with alkaline phosphatase coupled to avidin). These methods are simple to perform, specific for each isotype, reproducible with an average coefficient of variation of 5% for IgG1, 3% for IgG2a, 7% for IgG2b, 10% for IgG3, 3% for IgA and 7% for IgM, and at least 100 times more sensitive than radial immunodiffusion. The assays have been used to determine the absolute concentrations of mouse serum heavy chain Ig isotypes.
Similar articles
-
Serum concentrations of IgM, IgG1, IgG2b, IgG3 and IgA in C57BL/6 mice and their congenics at the nu(nude)locus.Thymus. 1990 Aug;16(1):45-54. Thymus. 1990. PMID: 2219232
-
Serum concentrations of IgM, IgG1, IgG2b, IgG3 and IgA in C57BL/6 mice and their congenics at the lpr (lymphoproliferation) locus.J Autoimmun. 1989 Dec;2(6):869-75. doi: 10.1016/0896-8411(89)90013-9. J Autoimmun. 1989. PMID: 2619870
-
Quantitation of monoclonal antibodies by ELISA. The use of purified mouse IgG and mouse IgM monoclonal antibodies as standards in a quantitative ELISA measuring monoclonal antibodies produced by cell culture.J Immunol Methods. 1993 Jun 4;162(1):77-83. doi: 10.1016/0022-1759(93)90409-z. J Immunol Methods. 1993. PMID: 8509654
-
The use of a double antibody sandwich ELISA and monoclonal antibodies for the assessment of porcine IgM, IgG and IgA concentrations.Vet Immunol Immunopathol. 1995 Feb;44(3-4):309-17. doi: 10.1016/0165-2427(94)05307-e. Vet Immunol Immunopathol. 1995. PMID: 7747408
-
An indirect asymmetrical sandwich ELISA using anti-allotype antibodies for the specific and quantitative measurement of mouse IgG2a of Igh-1b allotype.J Immunol Methods. 1989 Dec 20;125(1-2):207-13. doi: 10.1016/0022-1759(89)90095-1. J Immunol Methods. 1989. PMID: 2607153
Cited by
-
Descriptive Comparison of ELISAs for the Detection of Toxoplasma gondii Antibodies in Animals: A Systematic Review.Pathogens. 2021 May 15;10(5):605. doi: 10.3390/pathogens10050605. Pathogens. 2021. PMID: 34063342 Free PMC article. Review.
-
The immunoevasive function encoded by the mouse cytomegalovirus gene m152 protects the virus against T cell control in vivo.J Exp Med. 1999 Nov 1;190(9):1285-96. doi: 10.1084/jem.190.9.1285. J Exp Med. 1999. PMID: 10544200 Free PMC article.
-
Adoptive transfer of viable motheaten (mev) humoral autoimmunity: IgM to IgG switch of the hyperglobulinaemia in nude, beige recipient mice.Immunology. 1990 Nov;71(3):341-6. Immunology. 1990. PMID: 2269472 Free PMC article.
-
Adoptive transfer of viable motheaten humoral autoimmunity in cyclophosphamide-immunodepressed beige recipient mice.Immunology. 1990 Aug;70(4):520-6. Immunology. 1990. PMID: 2394466 Free PMC article.
-
Alymphoplasia (aly)-type nuclear factor kappaB-inducing kinase (NIK) causes defects in secondary lymphoid tissue chemokine receptor signaling and homing of peritoneal cells to the gut-associated lymphatic tissue system.J Exp Med. 2000 May 1;191(9):1477-86. doi: 10.1084/jem.191.9.1477. J Exp Med. 2000. PMID: 10790423 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
