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Comparative Study
. 2014 Sep;7(5):424-33.
doi: 10.1111/1751-7915.12131. Epub 2014 Jun 25.

Comparison of bacterial community structure and dynamics during the thermophilic composting of different types of solid wastes: anaerobic digestion residue, pig manure and chicken manure

Caihong Song  1 , Mingxiao Li, Xuan Jia, Zimin Wei, Yue Zhao, Beidou Xi, Chaowei Zhu, Dongming Liu
Affiliations
Comparative Study

Comparison of bacterial community structure and dynamics during the thermophilic composting of different types of solid wastes: anaerobic digestion residue, pig manure and chicken manure

Caihong Song et al. Microb Biotechnol. 2014 Sep.

Erratum in

Abstract

This study investigated the impact of composting substrate types on the bacterial community structure and dynamics during composting processes. To this end, pig manure (PM), chicken manure (CM), a mixture of PM and CM (PM + CM), and a mixture of PM, CM and anaerobic digestion residue (ADR) (PM + CM + ADR) were selected for thermophilic composting. The bacterial community structure and dynamics during the composting process were detected and analysed by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) coupled with a statistic analysis. The physical-chemical analyses indicated that compared to single-material composting (PM, CM), co-composting (PM + CM, PM + CM + ADR) could promote the degradation of organic matter and strengthen the ability of conserving nitrogen. A DGGE profile and statistical analysis demonstrated that co-composting, especially PM + CM + ADR, could improve the bacterial community structure and functional diversity, even in the thermophilic stage. Therefore, co-composting could weaken the screening effect of high temperature on bacterial communities. Dominant sequencing analyses indicated a dramatic shift in the dominant bacterial communities from single-material composting to co-composting. Notably, compared with PM, PM + CM increased the quantity of xylan-degrading bacteria and reduced the quantity of human pathogens.

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Figures

Figure 1
Figure 1
Temperature dynamics of PM, CM, the mixture of PM and CM (PM + CM), and the mixture of PM, CM and ADR (PM + CM + ADR). Arrows indicate each time of turning over. Error bars: mean ± standard deviation of two replicates.
Figure 2
Figure 2
The pH dynamics of PM, CM, the mixture of PM and CM (PM + CM), and the mixture of PM, CM and ADR (PM + CM + ADR). Error bars: mean ± standard deviation of two replicates.
Figure 3
Figure 3
DGGE profile of 16S rDNA gene fragments amplified with the primer set R534-P3.The numbers above each lane indicate the sampling months. Band patterns of PM, CM, PM + CM and PM + CM + ADR are presented. The enclosed bands and names indicate the clone names described in Table 2.
Figure 4
Figure 4
PCA of the 16S rDNA gene band patterns of PM, CM, PM + CM and PM + CM + ADR. Numbers beside the symbols indicate the sample names: 1, PM 0; 2, PM 1; 3, PM 2; 4, PM 3; 5, PM + CM + ADR 0; 6, PM + CM + ADR 1; 7, PM + CM + ADR 2; 8, PM + CM + ADR 3; 9, PM + CM 0; 10, PM + CM 1; 11, PM + CM 2; 12, PM + CM 3; 13, CM 0; 14, CM 1; 15, CM 2; 16, CM 3. The numbers after the name of composting treatment indicate the sampling months.
Figure 5
Figure 5
Similarity cluster analyses based on Complete Linkage algorithm for DGGE profiles for bacteria present in PM, CM, PM + CM and PM + CM + ADR. The numbers (0, 1, 2 and 3) beside composting treatment indicate the sampling months.
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