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. 2014 May;17(5):370-7.

Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran

Abdolvahab Moradi  1 , Sareh Zhand  1 , Amir Ghaemi  1 , Naeme Javid  1 , Masoud Bazouri  1 , Alijan Tabarraei  1
Affiliations

Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran

Abdolvahab Moradi et al. Iran J Basic Med Sci. 2014 May.

Abstract

Objectives: It has been reported that the mutation of the pre-core (PC) and basal-core promoter (BCP) may play an important role in the development of HBV-related hepatocellular carcinoma (HCC). In this study the PC and BCP mutations were investigated in chronic HBV patients.

Materials and methods: In this study, 120 chronic HBV patients from Golestan, Northeast of Iran who were not vaccinated against HBV, were recruited from the year 2008 to 2012. HBV-DNA extraction from plasma and PCR were performed and positive PCR products were subjected to automated sequencing.

Results: One hundred out of 120 (83.3%) patients were HBeAg negative. Comparison of our nucleotide sequences with reference sequence showed high rate mutation in BCP and PC region (96.66%). Frame shift mutation was found in 78 (65%) of patients in BCP region, among them 8 (6.6%) patients showed mutation in PC region.

Conclusion: Our results demonstrated high rate of mutations in BCP and PC regions among HBV chronic patients in Northeast of Iran.

Keywords: BCP mutation; Hepatitis B; Iran; PC mutation.

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Figures

Figure 1
Figure 1
Frequency and distribution of amino acid substitution in X gene
Figure 2
Figure 2
Frequency and distribution of amino acid substitution in PC region
See this image and copyright information in PMC

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