Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Mar 11:2013:612675.
doi: 10.5402/2013/612675. eCollection 2013.

Sex-Related Difference in Nitric Oxide Metabolites Levels after Nephroprotectant Supplementation Administration against Cisplatin-Induced Nephrotoxicity in Wistar Rat Model: The Role of Vitamin E, Erythropoietin, or N-Acetylcysteine

Mehdi Nematbakhsh  1 , Zahra Pezeshki  2
Affiliations

Sex-Related Difference in Nitric Oxide Metabolites Levels after Nephroprotectant Supplementation Administration against Cisplatin-Induced Nephrotoxicity in Wistar Rat Model: The Role of Vitamin E, Erythropoietin, or N-Acetylcysteine

Mehdi Nematbakhsh et al. ISRN Nephrol. .

Abstract

Background. Nitric oxide (NO) concentration in serum is altered by cisplatin (CP), and NO influences CP-induced nephrotoxicity. The effect of nephroprotectant agent supplementation (vitamin E, human recombinant erythropoietin (EPO), or n-acetylcysteine (NAC)) on the NO metabolites levels after CP administration in the two genders was determined. Methods. Sixty-four adult Wistar rats were randomly divided into 10 groups. Male and female rats in different groups received vehicle (saline), CP (7 mg/kg) alone, CP plus EPO (100 IU/kg), CP plus vitamin E (250 mg/kg), and CP plus NAC (600 mg/kg). CP was administrated as a single dose, but the supplementations were given for a period of 7 days. Results. In male rats, the serum levels of total NO metabolites (NO x ) and nitrite were increased significantly (P < 0.05) by CP. However, vitamin E significantly reduced the serum levels of these metabolites, which was increased by administration of CP (P < 0.05), and such findings were not observed for female rats. The EPO or NAC did not influence NO metabolites neither in male rats nor in female rats. Conclusion. Although vitamin E, EPO, and NAC are reported to be nephroprotectant agents against CP-induced nephrotoxicity, only vitamin E could reduce the level of all NO metabolites only in male rats.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Effect of EPO, vitamin E, and NAC on CP-increased serum levels of NOx, nitrate, and nitrite in male and female rats. The EPO, vitamin E, and NAC groups were compared with positive control group. (*) shows significant difference from control group (P < 0.05).
See this image and copyright information in PMC

References

    1. Eshraghi-Jazi F, Nematbakhsh M, Nasri H, et al. The protective role of endogenous nitric oxide donor (L-arginine) in cisplatin-induced nephrotoxicity: gender related differences in rat model. Journal of Research in Medical Sciences. 2011;16(11):1389–1396. - PMC - PubMed
    1. Saleh S, Ain-Shoka AA, El-Demerdash E, Khalef MM. Protective effects of the angiotensin II receptor blocker losartan on cisplatin-induced kidney injury. Chemotherapy. 2009;55(6):399–406. - PubMed
    1. Saleh S, El-Demerdash E. Protective effects of L-arginine against cisplatin-induced renal oxidative stress and toxicity: role of nitric oxide. Basic and Clinical Pharmacology and Toxicology. 2005;97(2):91–97. - PubMed
    1. Eguchi R, Fujimori Y, Ohta T, Kunimasa K, Nakano T. Calpain is involved in cisplatin-induced endothelial injury in an in vitro three-dimensional blood vessel model. International Journal of Oncology. 2010;37(5):1289–1296. - PubMed
    1. Nuver J, de Haas EC, van Zweeden M, Gietema JA, Meijer C. Vascular damage in testicular cancer patients: a study on endothelial activation by bleomycin and cisplatin in vitro. Oncology Reports. 2010;23(1):247–253. - PubMed

LinkOut - more resources