Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2014 Jul;52(7):2740-1.
doi: 10.1128/JCM.00962-14.

Use of reverse transcription-PCR-based assays for quantification of HIV-1 in dried blood spots requires specific HIV-1 RNA isolation for monitoring of antiretroviral treatment efficiency

François Rouet  1 , Dramane Kania  2 , Edouard Tuaillon  3 , Almoustapha Maiga  4 , Janin Nouhin  5 , Christine Rouzioux  6 , Diane Descamps  7 , Jean-Christophe Plantier  8
Affiliations
Comment

Use of reverse transcription-PCR-based assays for quantification of HIV-1 in dried blood spots requires specific HIV-1 RNA isolation for monitoring of antiretroviral treatment efficiency

François Rouet et al. J Clin Microbiol. 2014 Jul.
No abstract available

PubMed Disclaimer

Comment on

References

    1. Monleau M, Aghokeng AF, Eymard-Duvernay S, Dagnra A, Kania D, Ngo-Giang-Huong N, Toure-Kane C, Truong LX, Chaix ML, Delaporte E, Ayouba A, Peeters M. 2014. Field evaluation of dried blood spots for routine HIV-1 viral load and drug resistance monitoring in patients receiving antiretroviral therapy in Africa and Asia. J. Clin. Microbiol. 52:578–586. 10.1128/JCM.02860-13 - DOI - PMC - PubMed
    1. Waters L, Kambugu A, Tibenderana H, Meya D, John L, Mandalia S, Nabankema M, Namugga I, Quinn TC, Gazzard B, Reynolds SJ, Nelson M. 2007. Evaluation of filter paper transfer of whole-blood and plasma samples for quantifying HIV RNA in subjects on antiretroviral therapy in Uganda. J. Acquir. Immune Defic. Syndr. 46:590–593. 10.1097/QAI.0b013e318159d7f4 - DOI - PubMed
    1. Rouzioux C, Melard A, Avettand-Fenoel V. 2014. Quantification of total HIV1-DNA in peripheral blood mononuclear cells. Methods Mol. Biol. 1087:261–270. 10.1007/978-1-62703-670-2_21 - DOI - PubMed
    1. Mercier-Delarue S, Vray M, Plantier JC, Maillard T, Adjout Z, de Olivera F, Schnepf N, Maylin S, Simon F, Delaugerre C. 2014. Higher specificity of nucleic acid sequence-based amplification isothermal technology than of real-time PCR for quantification of HIV-1 RNA on dried blood spots. J. Clin. Microbiol. 52:52–56. 10.1128/JCM.01848-13 - DOI - PMC - PubMed
    1. Viljoen J, Gampini S, Danaviah S, Valea D, Pillay S, Kania D, Meda N, Newell ML, Van de Perre P, Rouet F. 2010. Dried blood spot HIV-1 RNA quantification using open real-time systems in South Africa and Burkina Faso. J. Acquir. Immune Defic. Syndr. 55:290–298. 10.1097/QAI.0b013e3181edaaf5 - DOI - PubMed

LinkOut - more resources