Concepts in quantitative fluorescence microscopy

Abstract

In recent years, there has been an enormous increase in the publication of spatial and temporal measurements made on fluorescence microscopy digital images. Quantitative fluorescence microscopy is a powerful and important tool in biological research but is also an error-prone technique that requires careful attention to detail. In this chapter, we focus on general concepts that are critical to performing accurate and precise quantitative fluorescence microscopy measurements.

Keywords: Fluorescence; Intensity; Measure; Microscopy; Quantify; Quantitate; Quantitation; Resolution; Sampling.