Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Jun 29:20:1095-100.
doi: 10.12659/MSM.890468.

The assessment of sIgA, histatin-5, and lactoperoxidase levels in saliva of adolescents with dental caries

Agnieszka Gornowicz  1 , Grażyna Tokajuk  2 , Anna Bielawska  1 , Elżbieta Maciorkowska  3 , Robert Jabłoński  3 , Anna Wójcicka  4 , Krzysztof Bielawski  5
Affiliations

The assessment of sIgA, histatin-5, and lactoperoxidase levels in saliva of adolescents with dental caries

Agnieszka Gornowicz et al. Med Sci Monit. .

Abstract

Background: Saliva contains a number of protective factors such as mucins, immunoglobulins (e.g., IgA, IgG, and IgM), and enzymes (e.g., lysozyme and lactoperoxidases) that play an important role in the maintenance of oral health. The aim of this study was to compare levels of sIgA, histatin-5, and lactoperoxidase in saliva of adolescents with dental caries.

Material and methods: Thirty-five adolescents (age 18 years) from high school were examined. Eight subjects with DMF=3 (Group I) and 27 adolescents with DMF>11 (Group II) were enrolled for this study. Clinical evaluation procedures comprised oral examination (including tooth, periodontal, and oral mucosal status) and collection of saliva samples. Saliva was collected for enzyme-linked immunosorbent assay (ELISA) and was used for determination of sIgA, histatin-5, and lactoperoxidase levels.

Results: Our results showed that adolescents with very high intensity of dental caries (DMF>11) had increased levels of sIgA, histatin-5, and lactoperoxidase compared to adolescents with lower intensity of caries. The increase was statistically significant (p<0.05).

Conclusions: We suggest that high intensity of caries is associated with increased levels of some salivary components - sIgA, histatin-5 and lactoperoxidase - that possess strong bactericidal or bacteriostatic effects, resulting in aggregation of oral bacteria and their clearance from the oral cavity.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Salivary concentration of sIgA in Group I (with DMF=3) and Group II (with DMF>11) using ELISA technique. ** P<0.05 – differences statistically significant between Group I and Group II.
Figure 2
Figure 2
Salivary concentration of histatin-5 in Group I (with DMF=3) and Group II (with DMF>11) using ELISA technique. ** P<0.05 – differences statistically significant between Group I and Group II.
Figure 3
Figure 3
Salivary concentration of LPO in Group I (with DMF=3) and Group II (with DMF>11) using technique ELISA. ** P<0.05 – differences statistically significant between Group I and Group II.
See this image and copyright information in PMC

References

    1. Jafarzadeh A, Sadeghi M, Karam GA, Vazirinejad R. Salivary IgA and IgE levels in healthy subjects: relation to age and gender. Braz Oral Res. 2010;24:21–27. - PubMed
    1. Featherstone JD. The continuum of dental caries-evidence for a dynamic disease process. J Dent Res. 2004;83:39–42. - PubMed
    1. Kuriakose S, Sundaresan C, Mathai V, et al. A comparative study of salivary buffering capacity, flow rate, resting pH, and salivary immunoglobulin A in children with rampant caries and caries-resistant children. J Indian Soc Pedod Prev Dent. 2013;31:69–73. - PubMed
    1. Ranadheer E, Nayak UA, Reddy NV, Rao VA. The relationship between salivary IgA levels and dental caries in children. J Indian Soc Pedod Prev Dent. 2011;29:106–12. - PubMed
    1. Chawda JG, Chaduvula N, Patel HR, et al. Salivary sIgA and dental caries activity. Indian Pediatr. 2011;48:719–21. - PubMed

Publication types