Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Oct;134(4):967-9.e7.
doi: 10.1016/j.jaci.2014.05.009. Epub 2014 Jun 27.

IgE antibodies to mountain cedar pollen predominantly recognize multiple conformational epitopes on Jun a 1

Randall M Goldblum  1 , Bo Ning  1 , Mark A Endsley  1 , D Mark Estes  1 , Barbara M Judy  1 , Julius van Bavel  2 , Terumi Midoro-Horiuti  3
Affiliations

IgE antibodies to mountain cedar pollen predominantly recognize multiple conformational epitopes on Jun a 1

Randall M Goldblum et al. J Allergy Clin Immunol. 2014 Oct.
No abstract available

PubMed Disclaimer

Conflict of interest statement

Disclosure of potential conflict of interest: R. M. Goldblum has received grants from the National Institutes of Health and has received consulting fees, travel support, and payment for lectures from Merck. B. Ning has a patent for antibody-mediated modulation of allergy. T. Midoro-Horiuti has received a grant from the National Institutes of Health (K08AI055792); has received consulting fees, travel support, and payment for lectures from Merck; and has a patent for antibody-mediated modulation of allergy. The rest of the authors declare that they have no relevant conflicts of interest.

Figures

FIG 1
FIG 1
Immunoglobulin binding to conformational and linear epitopes of Jun a 1. The % of binding due to discontinuous epitopes is indicated for each serum. These were computed from the relative amount of native (◆) and guanidine-denatured Jun a 1 (○) required to inhibit by 50% the binding of patient IgE to native Jun a 1 on ELISA plates (A) and to induce mast cell degranulation of RBL SX-38 cells sensitized with patient sera (B). RBL, Rat basophilic leukemia.
FIG 2
FIG 2
Relative binding of patients’ antibodies to discontinuous epitopes defined by mAbs G1 to G4. Binding of serum IgE from 7 patients with cedar pollinosis (a–g) and (A) mean of 7 patients to 4 distinct discontinuous epitopes of Jun a 1 (B). The results indicate the frequency and relative intensity of the patients’ responses to each of the 4 epitopes defined by mAbs G1 to G4.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Ivanciuc O, Midoro-Horiuti T, Schein CH, Xie L, Hillman GR, Goldblum RM, et al. The property distance index PD predicts peptides that cross-react with IgE antibodies. Mol Immunol. 2009;46:873–83. - PMC - PubMed
    1. Ivanciuc O, Garcia T, Torres M, Schein CH, Braun W. Characteristic motifs for families of allergenic proteins. Mol Immunol. 2009;46:559–68. - PMC - PubMed
    1. Radauer C, Breiteneder H. Pollen allergens are restricted to few protein families and show distinct patterns of species distribution. J Allergy Clin Immunol. 2006;117:141–7. - PubMed
    1. Liu Z, Bhattacharyya S, Ning B, Midoro-Horiuti T, Czerwinski EW, Goldblum RM, et al. Plant-expressed recombinant mountain cedar allergen Jun a 1 is allergenic and has limited pectate lyase activity. Int Arch Allergy Immunol. 2010;153:347–58. - PMC - PubMed
    1. Midoro-Horiuti T, Goldblum RM, Kurosky A, Goetz DW, Brooks EG. Isolation and characterization of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1. J Allergy Clin Immunol. 1999;104:608–12. - PubMed

Publication types

MeSH terms