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. 2014 Jul;78(3):161-7.

Increase in gene-transcript levels as indicators of up-regulation of the unfolded protein response in spontaneous canine tumors

Kirsten Elliot  1 , Valerie MacDonald-Dickinson  1 , Kathleen Linn  1 , Elemir Simko  1 , Vikram Misra  1
Affiliations

Increase in gene-transcript levels as indicators of up-regulation of the unfolded protein response in spontaneous canine tumors

Kirsten Elliot et al. Can J Vet Res. 2014 Jul.

Abstract
in English, French

The unfolded protein response (UPR), a conserved cellular response to stressors such as hypoxia and nutrient deprivation, is associated with angiogenesis and metastasis in tumor cells. This article discusses a pilot study conducted to determine whether components of the UPR could be identified in spontaneous canine tumors and whether they were up-regulated within tumor tissue compared with adjacent normal tissue. Tissue samples of various spontaneous canine neoplasms were taken from 13 dogs shortly after surgical excision or euthanasia; control samples were taken from adjacent normal tissue. RNA purification and real-time quantitative reverse-transcription polymerase chain reaction were done to measure the expression of 4 genes associated with the UPR (HERP, CHOP, GRP78, and XBP1s). The results indicated that UPR gene expression can be identified in spontaneous canine tumors and that the UPR is up-regulated, as indicated by significantly increased expression of CHOP and GRP78 within the tumor.

La réponse de la protéine non-repliée (UPR), une réponse cellulaire conservée à des stress tels que l’hypoxie et la privation de nutriments, est associée avec l’angiogénèse et les métastases chez les cellules tumorales. Cet article rapporte une étude pilote menée afin de déterminer si les composantes de l’UPR pourraient être identifiées dans des tumeurs canines spontanées et si elles sont surproduites à l’intérieur du tissu tumoral comparativement au tissu normal adjacent. Des échantillons de tissu provenant de divers néoplasmes spontanés canins ont été prélevés de 13 chiens peu de temps après exérèse chirurgicale ou euthanasie; des échantillons témoins ont été pris de tissu normal adjacent. La purification de l’ARN et une réaction d’amplification en chaine en temps réel quantitative utilisant la transcriptase réverse furent réalisées afin de mesurer l’expression de quatre gènes associés avec l’UPR (HERP, CHOP, GRP78, et XBP1s). Les résultats ont montré que l’expression du gène UPR peut être identifiée dans des tumeurs spontanées canines et que l’UPR est surproduit, tel que l’indique l’augmentation significative de l’expression de CHOP et GRP78 à l’intérieur de la tumeur.(Traduit par Docteur Serge Messier).

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Figures

Figure 1
Figure 1
Tissue from sample set number 2, confirming the diagnosis of hemangiosarcoma. a) Tumor margins consisting of normal skeletal muscle and fat: left, stained with hematoxylin and eosin (H&E); right, positive immunohistochemical staining of endothelium for factor-VIII-related antigen. b) Tumor mass: left, stained with H&E; right, moderate positive staining of neoplastic cells for factor-VIII-related antigen.
See this image and copyright information in PMC

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