Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Jul 16;136(28):9866-9.
doi: 10.1021/ja504845f. Epub 2014 Jul 1.

Liposomal spherical nucleic acids

Resham J Banga  1 , Natalia Chernyak, Suguna P Narayan, SonBinh T Nguyen, Chad A Mirkin
Affiliations

Liposomal spherical nucleic acids

Resham J Banga et al. J Am Chem Soc. .

Abstract

A novel class of metal-free spherical nucleic acid nanostructures was synthesized from readily available starting components. These particles consist of 30 nm liposomal cores, composed of an FDA-approved 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid monomer. The surface of the liposomes was functionalized with DNA strands modified with a tocopherol tail that intercalates into the phospholipid layer of the liposomal core via hydrophobic interactions. The spherical nucleic acid architecture not only stabilizes these constructs but also facilitates cellular internalization and gene regulation in SKOV-3 cells.

PubMed Disclaimer

Figures

Scheme 1
Scheme 1. Assembly of Liposomal SNAs from DOPC SUVs and Tocopherol-Modified DNA
Figure 1
Figure 1
(A) DLS of SUVs after purification. (B) DLS of liposomal SNAs after purification.
Figure 2
Figure 2
Stability studies of SUV and liposomal SNAs. Change in average diameter of SUVs (A) and liposomal SNAs (B) before (top) and after (bottom) heating in buffer, as measured by DLS. (C) Schematic representation of the decomposition of rhodamine-encapsulated liposome in the presence of bovine serum albumin, a major component of FBS. (D) Degradation of SUVs (red traces) and liposomal SNAs (blue traces) in the presence of 10% FBS, as measured by increases in the fluorescence intensity of rhodamine.
Figure 3
Figure 3
(A) Extinction spectra of liposomal SNAs before (blue) and after (red) aggregation in the presence of linker DNA strands. (B) Melting transition of liposomal SNA aggregates monitored as a change in extinction at 260 nm.
Figure 4
Figure 4
(A) Confocal micrograph of SKOV-3 cells incubated with 100 nM Cy5-labeled liposomal SNAs (red) for 24 h. (B) Flow cytometry analysis of uptake of 5-Cy5-labeled DNA strand and 5′-Cy5-labeled liposomal SNAs in SKOV-3 cells after 1 h (blue bars) and 36 h (green bars) of incubation. (C) Cytotoxicity (alamarBlue assay) of liposomal SNAs and DharmaFECT-delivered DNA in SKOV-3 cells. (D) HER2 gene knockdown in SKOV-3 cells using anti-HER2 liposomal SNA constructs at 1 μM DNA.
See this image and copyright information in PMC

References

    1. Mirkin C. A.; Letsinger R. L.; Mucic R. C.; Storhoff J. J. Nature 1996, 382, 607. - PubMed
    1. Cutler J. I.; Auyeung E.; Mirkin C. A. J. Am. Chem. Soc. 2012, 134, 1376. - PubMed
    2. Briley W.; Halo T. L.; Randeria P. S.; Alhasan A. H.; Auyeung E.; Hurst S. J.; Mirkin C. A. In Nanomaterials for Biomedicine; Nagarajan R., Ed.; ACS Symposium Series1119; American Chemical Society: Washington, DC, 2012; pp 1–20.
    1. Young K. L.; Scott A. W.; Hao L.; Mirkin S. E.; Liu G.; Mirkin C. A. Nano Lett. 2012, 12, 3867. - PMC - PubMed
    1. Cutler J. I.; Zheng D.; Xu X.; Giljohann D. A.; Mirkin C. A. Nano Lett. 2010, 10, 1477. - PMC - PubMed
    1. Lee J.-S.; Lytton-Jean A. K. R.; Hurst S. J.; Mirkin C. A. Nano Lett. 2007, 7, 2112. - PMC - PubMed

Publication types