Modern mass spectrometry-based structural proteomics

Abstract

Recent developments in the modern mass spectrometry of proteins and peptides have resulted in significant progress in structural proteomics techniques for studying protein structure. A variety of protein structural questions, ranging from defining protein interaction networks to the study of conformational changes and the structure of single proteins, can be addressed using multiple mass spectrometry-based structural proteomics approaches. Each technique provides specific structural information which can be used as experimental structural constraints in protein structure modeling. Here, we describe recent developments in limited proteolysis, surface modification, hydrogen-deuterium exchange, ion mobility, and cross-linking-all combined with modern mass spectrometric techniques-for the studying protein structure.

Keywords: Cross-linking; Hydrogen–deuterium exchange; Limited proteolysis; Mass spectrometry; Prion protein; Structural proteomics; Surface modification.