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. 2014:1178:101-10.
doi: 10.1007/978-1-4939-1016-8_10.

Eosinophil chemotaxis

Gordon Dent  1
Affiliations

Eosinophil chemotaxis

Gordon Dent. Methods Mol Biol. 2014.

Abstract

Chemotaxis assays have a number of applications in the study of leukocyte biology and immune/inflammatory pathology. Multiwell "blind chamber"-type assays allow a large number of parallel measurements within a single assay. The development of fluorescence assays using microplate-based chemotaxis chambers has permitted a degree of automation to be applied to these assays. Here, a method is described for the quantitative measurement of eosinophil migration using a 96-well assay with numbers of cells that may realistically be obtained from blood samples.

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