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Comment
. 2014 Jul 15;111(28):10029-30.
doi: 10.1073/pnas.1408985111. Epub 2014 Jul 2.

Fulfilling the dream of a perfect genome editing tool

Maria Spies  1
Affiliations
Comment

Fulfilling the dream of a perfect genome editing tool

Maria Spies. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1.
Fig. 1.
R-loop formation by Cascade and Cas9. Overall architectures of Cascade (Left) and Ca9 (Right) ribonucleoproteins and their respective R-loops. Although both complexes initiate R-loop formation by recognizing their respective PAM sequences flanking the protospacer, the Cascade reaction proceeds through a metastable intermediate, which is then locked to form a stable R-loop.
See this image and copyright information in PMC

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References

    1. Szczelkun MD, et al. Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes. Proc Natl Acad Sci USA. 2014;111(27):9798–9803. - PMC - PubMed
    1. Carroll D. Genome engineering with targetable nucleases. Annu Rev Biochem. 2014;83:409–439. - PubMed
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    1. Jinek M, et al. Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science. 2014;343(6176):1247997. - PMC - PubMed

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