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. 2014 Apr;10(Suppl 2):S318-23.
doi: 10.4103/0973-1296.133287.

Optimization of Ultrasound-assisted extraction of polyphenols, tannins and epigallocatechin gallate from barks of Stryphnodendron adstringens (Mart.) Coville bark extracts

Jordana N Sousa  1 , Nathália B Pedroso  1 , Leonardo L Borges  1 , Gerlon A R Oliveira  1 , José R Paula  1 , Edemilson C Conceição  1
Affiliations

Optimization of Ultrasound-assisted extraction of polyphenols, tannins and epigallocatechin gallate from barks of Stryphnodendron adstringens (Mart.) Coville bark extracts

Jordana N Sousa et al. Pharmacogn Mag. 2014 Apr.

Abstract

Background: Stryphnodendron adstringens (Mar.) Coville is a native plant from Brazil, rich in phenolic compounds and used on popular medicine as a wound healing agent, in the treatment of gastric lesions and as antimicrobial.

Materials and methods: Ultrassound-assisted extraction (UAE) was applied to extraction of epigallocatechin gallate (EGCG), total polyphenols (TP) and total tannins (TT) content from barks of Stryphnodendron adstringens (Mar.) Coville. Several operating parameters, namely extraction time (min), liquid to solid ratio (mg/mL), ethanolic strength (%, v/v), were optimized using response surface methodology (RSM) with a Box-Behnken design.

Results: By using the desirability function approach, the optimum UAE conditions to obtain desirable extraction yields for all these metabolites simultaneously were found at the extraction time of 30 min, solid to liquid ratio of 4 mg/mL and ethanolic strength of 65. Under these conditions, the epigallocatechin gallate, total polyphenols and total tannins content were 0.31; 22.95 and 11.95 % (w/w), respectively.

Conclusion: The results indicated that knowledge gained from this study should be helpful to further exploit and apply this resource and also showed the feasibility of ultrasound-assisted extraction for obtaining GEGC, TP and TT from barks of S. adstrigens.

Keywords: Desirability function; Stryphnodendron adstringens; optimization; response surface methodology; ultrassound-assisted extraction.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Response surface of EGCG as a function of extraction time (ET) and ethanolic strength (ES)
Figure 2
Figure 2
Response surface of TPc as a function of extraction time (ET) and solid liquid ratio (SLR)
Figure 3
Figure 3
Countour plot of Desirability as a function of extraction time (ET) and solid to liquid ratio (SLR) (a); extraction time (ET) and ethanolic strength (ES) (b); solid to liquid ratio (SLR) and ethanolic strength (ES) (c)
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