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. 2014 Sep-Oct;16(5):790-1.
doi: 10.4103/1008-682X.132979.

Isolation and characterization of detergent-resistant membranes from rat spermatozoa

Shuang-Gang Hu, Xiang-Qi Li, Chun-Hua Tang, Yun Sun  1 , Yong-Lian Zhang  2
Affiliations

Isolation and characterization of detergent-resistant membranes from rat spermatozoa

Shuang-Gang Hu et al. Asian J Androl. 2014 Sep-Oct.
No abstract available

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Figures

Figure 1
Figure 1
(a) Distribution of detergent-resistant membranes (DRMs) in the sucrose density gradient of rat sperm Triton X-100 lysate. Numbers on the right of the tube denote 1 ml fractions from top (No. 1) to bottom (No. 12) of the tube. Note that DRMs were localized at fraction No. 6 (appears as an opalescent band). (b) The total protein content of 12 membrane fractions was determined. The data are presented as the mean ± standard deviation of three replicates. (c) Caveolin-1 and flotillin-2 differentially target DRMs of rat spermatozoa. Sucrose density gradient fractions of 1% Triton X-100 extracted rat sperm lysates were analyzed for caveolin-1 and flotillin-2 immunoreactivity. Flotillin-2 floats in DRM fraction whereas caveolin-1 is found at the bottom of the gradient in the non-DRM fractions.
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