Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Sep-Oct;16(5):790-1.
doi: 10.4103/1008-682X.132979.

Isolation and characterization of detergent-resistant membranes from rat spermatozoa

Shuang-Gang Hu, Xiang-Qi Li, Chun-Hua Tang, Yun Sun  1 , Yong-Lian Zhang  2
Affiliations

Isolation and characterization of detergent-resistant membranes from rat spermatozoa

Shuang-Gang Hu et al. Asian J Androl. 2014 Sep-Oct.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
(a) Distribution of detergent-resistant membranes (DRMs) in the sucrose density gradient of rat sperm Triton X-100 lysate. Numbers on the right of the tube denote 1 ml fractions from top (No. 1) to bottom (No. 12) of the tube. Note that DRMs were localized at fraction No. 6 (appears as an opalescent band). (b) The total protein content of 12 membrane fractions was determined. The data are presented as the mean ± standard deviation of three replicates. (c) Caveolin-1 and flotillin-2 differentially target DRMs of rat spermatozoa. Sucrose density gradient fractions of 1% Triton X-100 extracted rat sperm lysates were analyzed for caveolin-1 and flotillin-2 immunoreactivity. Flotillin-2 floats in DRM fraction whereas caveolin-1 is found at the bottom of the gradient in the non-DRM fractions.
See this image and copyright information in PMC

References

    1. Brown DA, London E. Functions of lipid rafts in biological membranes. Annu Rev Cell Dev Biol. 1998;14:111–36. - PubMed
    1. Cross NL. Reorganization of lipid rafts during capacitation of human sperm. Biol Reprod. 2004;71:1367–73. - PubMed
    1. Nixon B, Bielanowicz A, McLaughlin EA, Tanphaichitr N, Ensslin MA, et al. Composition and significance of detergent resistant membranes in mouse spermatozoa. J Cell Physiol. 2009;218:122–34. - PubMed
    1. Bou Khalil M, Chakrabandhu K, Xu H, Weerachatyanukul W, Buhr M, et al. Sperm capacitation induces an increase in lipid rafts having zona pellucida binding ability and containing sulfogalactosylglycerolipid. Dev Biol. 2006;290:220–35. - PubMed
    1. Girouard J, Frenette G, Sullivan R. Seminal plasma proteins regulate the association of lipids and proteins within detergent-resistant membrane domains of bovine spermatozoa. Biol Reprod. 2008;78:921–31. - PubMed

Publication types