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. 2014 Jul;70(Pt 7):959-62.
doi: 10.1107/S2053230X14011844. Epub 2014 Jun 19.

Cloning, overexpression, purification and preliminary X-ray analysis of the protein kinase domain of enhanced disease resistance 1 (EDR1) from Arabidopsis thaliana

Heidi Kaljunen  1 , Saravanan Panneerselvam  2 , Jochen Mueller-Dieckmann  3
Affiliations

Cloning, overexpression, purification and preliminary X-ray analysis of the protein kinase domain of enhanced disease resistance 1 (EDR1) from Arabidopsis thaliana

Heidi Kaljunen et al. Acta Crystallogr F Struct Biol Commun. 2014 Jul.

Abstract

Enhanced disease resistance 1 is a member of the Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK) family that negatively regulates disease resistance, ethylene-induced senescence and programmed cell death in response to both abiotic and biotic stresses. A catalytically inactive form of the EDR1 kinase domain was successfully cloned, expressed, purified and crystallized. Crystallization was conducted in the presence of the ATP analogue AMP-PNP. The crystals belonged to space group P3221 and contained two molecules in the asymmetric unit. The crystals diffracted X-rays to 2.55 Å resolution.

Keywords: Arabidopsis thaliana; MAPKKK family; enhanced disease resistance 1.

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Figures

Figure 1
Figure 1
Crystals of the EDR1 D792N mutant from A. thaliana. The largest crystal shown here is about 300 × 20 × 20 µm.
See this image and copyright information in PMC

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