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. 2013 Dec;3(4):856-61.
doi: 10.1086/674764.

Oxidative-stress biomarkers in patients with pulmonary hypertension

Graziela S Reis  1 , Viviane S Augusto  1 , Ana Paula C Silveira  1 , Alceu A Jordão Jr  2 , José Baddini-Martinez  2 , Omero Poli Neto  1 , Alfredo José Rodrigues  1 , Paulo Roberto B Evora  1
Affiliations

Oxidative-stress biomarkers in patients with pulmonary hypertension

Graziela S Reis et al. Pulm Circ. 2013 Dec.

Abstract

This controlled, prospective, nonrandomized clinical investigation has as its chief strength the fact that it was done in humans with active disease and apparently on fairly modest therapeutic regimens. The aim was to present the results of oxidative-stress biomarkers in humans suffering from pulmonary artery hypertension (PAH). Inflammation and oxidative stress are essential in PAH with increased lipid peroxidation and reduced antioxidant defenses. Twenty-four adult patients of both sexes, with a mean age of 21 years, were subdivided into 2 groups: a control group of 12 healthy, nonsmoking volunteers and a PAH group (PAHG) of 12 volunteers with PAH receiving outpatient treatment. Oxidative stress was evaluated by plasma activity of reduced glutathione (GSH); lipid peroxidation was expressed by malondialdehyde (MDA) and lipid hydroperoxide (ferrous oxidation of xylenol orange [FOX] assay); vitamin E was measured by high-performance liquid chromatography and tumor necrosis factor-α (TNF-α) by enzyme-linked immunosorbent assay. Statistical analyses showed significant differences for (1) the TNF-α measure, with highest values in PAHG patients; (2) the plasma GSH, with lowest values in PAHG patients; (3) vitamin E, with the lowest concentrations in PAHG patients; (4) MDA measure, with highest values in PAHG patients; and (5) the lipid hydroperoxide FOX measure, with highest values in PAHG patients. In conclusion, inflammation and oxidative stress are present in patients with PAH, as confirmed by increased lipid peroxidation, reduced GSH, and low concentrations of vitamin E.

Keywords: oxidative stress; pulmonary circulation; pulmonary hypertension.

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Figures

Figure 1
Figure 1
Values of plasma malondialdehyde (MDA) in absolute numbers in the control group (CG) and the pulmonary hypertension group (PAHG). Results are expressed as median and 25% and 75% quartiles; N = 12.
Figure 2
Figure 2
Values of plasma tumor necrosis factor-α (TNF-α) in absolute numbers in the control group (CG) and the pulmonary hypertension group (PAHG). Results are expressed as median and 25% and 75% quartiles; N = 12.
Figure 3
Figure 3
Values of plasma vitamin E in absolute numbers in the control group (CG) and the pulmonary hypertension group (PAHG). Results are expressed as median and 25% and 75% quartiles; N = 12.
Figure 4
Figure 4
Values of plasma reduced glutathione (GSH) in absolute numbers in the control group (CG) and the pulmonary hypertension group (PAHG). Results are expressed as median and 25% and 75% quartiles; N = 12.
Figure 5
Figure 5
Values of plasma lipid hydroperoxide (ferrous oxidation of xylenol orange [FOX] assay) in absolute numbers in the control group (CG) and the pulmonary hypertension group (PAHG). Results are expressed as median and 25% and 75% quartiles; N = 12.
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References

    1. Bottje W, Enkvetchakul B, Moore R, McNew R. Effect of α-tocopherol on antioxidants, lipid peroxidation, and the incidence of pulmonary hypertension syndrome (ascites) in broilers. Poult Sci 1995;74:1356–1369. - PubMed
    1. Streb JW, Miano JM. Retinoids: pleiotropic agents of therapy for vascular diseases? Curr Drug Targets Cardiovasc Haematol Disord 2003;3:31–57. - PubMed
    1. Cracowski JC, Cracowski C, Bessard G, Pepin JL, Bessard J, Schwebel C, Stanke-Labesque F, Pison C. Increased lipid peroxidation in patients with pulmonary hypertension. Am J Respir Crit Care Med 2001;164:1038–1042. - PubMed
    1. Gérard-Monnier D, Erdelmeier I, Régnard K, Moze-Henry N, Yadan JC, Chaudière J. Reactions of 1-methyl-2-phenylindole with malondialdehyde and 4-hydroxyalkenals: analytical applications to a colorimetric assay of lipid peroxidation. Chem Res Toxicol 1998;11:1176–1183. - PubMed
    1. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 1968;25:192–205. - PubMed

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