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Comparative transcriptome analysis reveals significant differences in MicroRNA expression and their target genes between adipose and muscular tissues in cattle
1 College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
2 Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, Jiangsu, China.
Comparative transcriptome analysis reveals significant differences in MicroRNA expression and their target genes between adipose and muscular tissues in cattle
1 College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
2 Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, Jiangsu, China.
The posttranscriptional gene regulation mediated by microRNAs (miRNAs) plays an important role in various species. However, to date limited miRNAs have been reported between fat and muscle tissues in beef cattle. In this paper, 412 known and 22 novel miRNAs in backfat as well as 334 known and 10 novel miRNAs in longissimus thoracis were identified in the Chinese Qinchuan beef cattle. Bta-miR-199a-3p, -154c, -320a and -432 were expressed at higher levels in backfat tissue, while bta-miR-1, -133a, -206, and -378 were also significantly enriched in muscle tissue. Functional analysis revealed that fat-enriched miRNAs targeted PRKAA1/2, PPARA and PPARG genes to modulate lipid and fatty acid metabolism, and muscle-enriched miRNAs targeted CSRP3 gene to present function involved in skeletal and muscular system development. The results obtained may help in the design of new selection strategies to improve beef quality.
Competing Interests: The authors have declared that no competing interests exist.
Figures
Figure 1. Summary of the common and…
Figure 1. Summary of the common and specific tags of two samples, including the summary…
Figure 1. Summary of the common and specific tags of two samples, including the summary of unique tags (A) and total tags (B).
Figure 2. Length distribution of small RNAs…
Figure 2. Length distribution of small RNAs in AF and AM libraries.
Figure 2. Length distribution of small RNAs in AF and AM libraries.
Figure 3. Distribution of the genome-mapped sequence…
Figure 3. Distribution of the genome-mapped sequence reads in AF (A) and AM (B) small…
Figure 3. Distribution of the genome-mapped sequence reads in AF (A) and AM (B) small RNA libraries.
Figure 4. The differential expression of bovine…
Figure 4. The differential expression of bovine conserved (A) and novel (B) miRNAs between AF…
Figure 4. The differential expression of bovine conserved (A) and novel (B) miRNAs between AF and AM tissue were shown.
Note: Expression level (AF): Expression level of adult bovine backfat tissue; Expression level (AM): Expression level of adult bovine muscle tissue. Each point in the figure represents a miRNA. Red points represent miRNAs with a fold change >2, blue points represent miRNAs with 1/2
Figure 5. The expression of miRNAs in…
Figure 5. The expression of miRNAs in bovine different tissues were detected by RT-qPCR.
Figure 5. The expression of miRNAs in bovine different tissues were detected by RT-qPCR.
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