Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Apr;8(4):1087-92.
doi: 10.1002/j.1460-2075.1989.tb03477.x.

The mammalian G protein rhoC is ADP-ribosylated by Clostridium botulinum exoenzyme C3 and affects actin microfilaments in Vero cells

P Chardin  1 , P Boquet, P Madaule, M R Popoff, E J Rubin, D M Gill
Affiliations

The mammalian G protein rhoC is ADP-ribosylated by Clostridium botulinum exoenzyme C3 and affects actin microfilaments in Vero cells

P Chardin et al. EMBO J. 1989 Apr.

Abstract

Clostridium botulinum C3 is a recently discovered exoenzyme that ADP-ribosylates a eukaryotic GTP-binding protein of the ras superfamily. We show now that the bacterially-expressed product of the human rhoC gene is ADP-ribosylated by C3 and corresponds in size, charge and behavior to the dominant C3 substrate of eukaryotic cells. C3 treatment of Vero cells results in the disappearance of microfilaments and in actinomorphic shape changes without any apparent direct effect upon actin. Thus the ADP-ribosylation of a rho protein seems to be responsible for microfilament disassembly and we infer that the unmodified form of a rho protein may be involved in cytoskeletal control.

PubMed Disclaimer

References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Biol Chem. 1988 Nov 5;263(31):16303-8 - PubMed
    1. Biochemistry. 1976 Feb 10;15(3):616-23 - PubMed
    1. J Biol Chem. 1977 Feb 10;252(3):1102-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Jan;80(1):21-5 - PubMed

Publication types