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. 1989 Jun 26;17(12):4689-98.
doi: 10.1093/nar/17.12.4689.

Poly(ADP-ribose)polymerase: a novel finger protein

A Mazen  1 , J Menissier-de Murcia, M Molinete, F Simonin, G Gradwohl, G Poirier, G de Murcia
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Free PMC article

Poly(ADP-ribose)polymerase: a novel finger protein

A Mazen et al. Nucleic Acids Res. .
Free PMC article

Abstract

By Energy Dispersive X-ray fluorescence we have determined that calf thymus poly(ADP-ribose) polymerase binds two zinc ions per enzyme molecule. Using 65Zn (II) for detection of zinc binding proteins and polypeptides on western blots, we found that the zinc binding sites are localized in a 29 kd N-terminal fragment which is included in the DNA binding domain. Metal depletion and restoration experiments proved that zinc is essential for the binding of this fragment to DNA as tested by Southwestern assay. These results correlate with the existence of two putative zinc finger motifs present in the N-terminal part of the human enzyme. Poly(ADP-ribose)polymerase fingers could be involved in the recognition of DNA strand breaks and therefore in enzyme activation.

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