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. 2014 Oct:105:102-4.
doi: 10.1016/j.mimet.2014.07.010. Epub 2014 Jul 11.

Evaluation of fluorescent stains for visualizing extracellular DNA in biofilms

Mira Okshevsky  1 , Rikke Louise Meyer  2
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Evaluation of fluorescent stains for visualizing extracellular DNA in biofilms

Mira Okshevsky et al. J Microbiol Methods. 2014 Oct.

Abstract

Here we determine an optimal technique for the visualization of extracellular DNA in bacterial biofilms using the fluorescent eDNA stain TOTO-1 and the counterstain SYTO 60. This technique allows for more sensitive eDNA visualization than other fluorescent staining methods currently in use.

Keywords: Biofilm; Extracellular DNA; Fluorescent staining; Imaging; eDNA.

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