[Expression of synthetic human angiogenin gene in E. coli in cleaved beta-galactosidase-angiogenin protein]
[Article in
Russian]
- PMID: 2502122
Item in Clipboard
[Expression of synthetic human angiogenin gene in E. coli in cleaved beta-galactosidase-angiogenin protein]
[Article in
Russian]
Bioorg Khim.
1989 Apr.
Abstract
A synthetic gene coding for human angiogenin was cloned in pUR290 plasmid in frame with beta-galactosidase, both parts of the resultant fused protein being joined through an Asp-Pro sequence. The fused protein, synthesised in E. coli cells upon IPTG induction and isolated as inclusion bodies, possessed angiogenic activity on the chick chorioallantois membrane and was cleaved upon acid treatment to yield free angiogenin.
Similar articles
-
[Expression of synthetic gene for human angiogenin in E. coli cells].Bioorg Khim. 1996 Dec;22(12):891-3. Bioorg Khim. 1996. PMID: 9054338 Russian.
-
[Expressing plasmid vectors on the basis of Escherichia coli beta-galactosidase gene fragments].Bioorg Khim. 1987 Mar;13(3):350-8. Bioorg Khim. 1987. PMID: 3036168 Russian.
-
[Expression of the hybrid protein beta-galactosidase-[Val8]-calcitonin in Escherichia coli].Dokl Akad Nauk SSSR. 1985;283(6):1507-9. Dokl Akad Nauk SSSR. 1985. PMID: 3933940 Russian. No abstract available.
-
Angiogenin, a potent mediator of angiogenesis. Biological, biochemical and structural properties.Pathol Biol (Paris). 1999 Apr;47(4):345-51. Pathol Biol (Paris). 1999. PMID: 10372403 Review.
-
Production of epidermal growth factor in Escherichia coli from a synthetic gene.J Cell Sci Suppl. 1985;3:29-38. doi: 10.1242/jcs.1985.supplement_3.4. J Cell Sci Suppl. 1985. PMID: 3938982 Review.