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. 2015 Jan;135(1):302-304.
doi: 10.1038/jid.2014.304. Epub 2014 Jul 18.

Talaporfin sodium-mediated photodynamic therapy alone and in combination with pulsed dye laser on cutaneous vasculature

Affiliations

Talaporfin sodium-mediated photodynamic therapy alone and in combination with pulsed dye laser on cutaneous vasculature

Kristen M Kelly et al. J Invest Dermatol. 2015 Jan.
No abstract available

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Conflict of interest statement

CONFLICT OF INTEREST

Light Sciences Oncology provided TS for this research.

Figures

Figure 1
Figure 1. The combination of TS-mediated PDT and PDL irradiation leads to a significant reduction in the characteristic PDT radiant exposure required to achieve persistent vascular shutdown
We first determined the characteristic radiant exposures associated with persistent vascular shutdown following 595-nm pulsed dye laser (PDL) irradiation. We performed PDL on the epidermal side of window chambers and imaged blood-flow using Laser Speckle Imaging (LSI). We assessed persistent vascular shutdown on Day 7. We assigned a “0” score if some evidence of blood flow was present, and a “1” score if flow was no longer evident. We used dose-response analysis to calculate a characteristic radiant exposure (RE50/7) at which 50% of irradiated window chambers are expected to have vascular shutdown on Day 7. (A–C) Representative LSI data associated with 595-nm PDL irradiation, in which persistent vascular shutdown was not (A,B) and was (C) achieved, using radiant exposures of (A) 4, (B) 6, and (C) 10 J/cm2, respectively. (D) Based on data from 19 experiments, we identified a RE50/7 of 7.1 J/cm2 for PDL irradiation. We then studied the combination of TS-mediated PDT and PDL irradiation. In this set of experiments, we used PDT (20 to 60 J/cm2) and PDL radiant exposures (4 to 6 J/cm2) that were below the RE50/7 values of 85 J/cm2 (Moy et al., 2012) and 7.1 J/cm2 (Figure 1D), respectfully. (E) Representative maps of blood flow that demonstrate persistent vascular shutdown at Day 7. In this specific example, we applied PDT (60 J/cm2) followed by PDL (6 J/cm2). This combination resulted in marked acute vascular shutdown, which persisted through Day 7. (F) Based on data from 30 experiments, we determined that the characteristic radiant exposure required to achieve persistent vascular shutdown, decreased from 85 J/cm2 with PDT alone to 45 J/cm2 for the combined PDT+PDL protocol. Scale bars = 2mm.

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