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. 2014 Aug 18:395:47-51.
doi: 10.1016/j.carres.2014.05.002. Epub 2014 May 15.

Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase

D Mangan  1 , B V McCleary  2 , A Liadova  2 , R Ivory  2 , N McCormack  2
Affiliations

Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase

D Mangan et al. Carbohydr Res. .

Abstract

There is a growing demand for research tools to aid the scientific community in the search for improved cellulase enzymes for the biofuel industry. In this work, we describe a novel fluorometric assay for cellulase (endo-1,4-β-glucanase) which is based on the use of 4,6-O-benzylidene-4-methylumbelliferyl-β-cellotrioside (BzMUG3) in the presence of an ancillary β-glucosidase. This assay can be used quantitatively over a reasonable linear range, or qualitatively as a solution screening tool which may find extensive use in the area of metagenomics.

Keywords: 4-Methylumbelliferone; Assay procedure; Cellulase; Fluorometric; Metagenomics; endo-1,4-β-Glucanase.

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