Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989:7 Suppl 1:208-11.

Structure of pAL5000, a plasmid from M. fortuitum and its utilization in transformation of mycobacteria

B Gicquel-Sanzey  1 , J Moniz-Pereira, M Gheorghiu, J Rauzier
Affiliations
  • PMID: 2503992

Structure of pAL5000, a plasmid from M. fortuitum and its utilization in transformation of mycobacteria

B Gicquel-Sanzey et al. Acta Leprol. 1989.

Abstract

We have developed a gene cloning system for mycobacteria. Based on the nucleotide sequence determined for the M. fortuitum plasmid pAL5000, we have constructed an E. coli/mycobacteria shuttle vector. This vector, pAL8, is composed of pAL5000, pTZ19R (an E. coli plasmid) and a kanamycin resistance gene (from Tn903). We were unable to obtain viable kanamycin resistant pAL8 transformants of M. smegmatis using a PEG-mediated DNA uptake system, in spite of the fact that we could show efficient DNA uptake by transfection using the mycobacterial lytic phage D29. However, kanamycin resistant transformants of M. smegmatis or BCG could be obtained by electroporation. This plasmid cloning system provides a tool for studies of the expression of cloned genes (e.g. virulence) or epitopes in mycobacteria and allows the rational construction of recombinant BCG polyvalent vaccines.

PubMed Disclaimer

Publication types