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. 2013 Spring;3(2):112-25.

Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves

Badrul Alam  1 , Fahima Akter  1 , Nahida Parvin  1 , Rashna Sharmin Pia  1 , Sharmin Akter  1 , Jesmin Chowdhury  1 , Kazi Sifath-E-Jahan  1 , Ekramul Haque  2
Affiliations

Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves

Badrul Alam et al. Avicenna J Phytomed. 2013 Spring.

Abstract

Objective: The present study was designed to evaluate the antioxidant, analgesic, and anti-inflammatory activities of the methanolic extract of Piper betle leaves (MPBL).

Materials and methods: MPBL was evaluated for anti-inflammatory activity using carrageenan-induced hind paw edema model. Analgesic activity of MPBL was evaluated by hot plate, writhing, and formalin tests. Total phenolic and flavonoids content, total antioxidant activity, scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, peroxynitrate (ONOO) as well as inhibition of total ROS generation, and assessment of reducing power were used to evaluate antioxidant potential of MPBL.

Results: The extract of MPBL, at the dose of 100 and 200 mg/kg, produced a significant (p<0.05) increase in pain threshold in hot plate method whereas significantly (p<0.05) reduced the writhing caused by acetic acid and the number of licks induced by formalin in a dose-dependent manner. The same ranges of doses of MPBL caused significant (p<0.05) inhibition of carrageenan-induced paw edema after 4 h in a dose-dependent manner. In DPPH, ONOO(-), and total ROS scavenging method, MPBL showed good antioxidant potentiality with the IC50 value of 16.33±1.02, 25.16±0.61 , and 41.72±0.48 µg/ml, respectively with a significant (p<0.05) good reducing power.

Conclusion: The findings of the study suggested that MPBL has strong analgesic, anti-inflammatory, and antioxidant effects, conforming the traditional use of this plant for inflammatory pain alleviation to its antioxidant potentiality.

Keywords: Analgesic; Anti-inflammatory; Antioxidant; Piper betle.

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Figures

Figure 1
Figure 1
Values are mean±SEM, Reducing power of MPBL, quercetin, ascorbic acid, and galic acid by spectrophotometric detection of Fe3+ to Fe2+ transformation
Figure 2
Figure 2
Effects of the MPBL on latency to hot plate test. Values are mean±SEM, (n=5); *p<0.05 as compared with vehicle control (one-way ANOVA followed by Dunnett’s test). Group I animals received vehicle (1% Tween 80 in water), Group II received nalbuphine 10 mg/kg body weight, Group III and Group IV were treated with 100 and 200 mg/kg body weight (p.o.) of the crude extract of P. betle, respectively
Figure 3
Figure 3
Effects of the MPBL on carrageenan-induced paw edema test. Values are mean±SEM, (n=5); *p<0.05 as compared to vehicle control (one-way ANOVA followed by Dunnett’s test). Group I animals received vehicle (1% Tween 80 in water), Group II received indomethacin10 mg/kg body weight, Group III and Group IV were treated with 100 and 200 mg/kg body weight (p.o.) of the crude extract of P. betle, respectively
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