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. 2014 Mar-Apr:(2):25-9.

[Measurement of genital mycoplasma concentration by using a microbiological and molecular-biological methods]

[Article in Russian]
T A Ivanova, A E Gushchin, N A Gamova, O I Barkhatova, I V Rakovskaia
  • PMID: 25051693

[Measurement of genital mycoplasma concentration by using a microbiological and molecular-biological methods]

[Article in Russian]
T A Ivanova et al. Zh Mikrobiol Epidemiol Immunobiol. 2014 Mar-Apr.

Abstract

Aim: Establishment of ratios that would allow to execute recalculation of mycoplasma concentration from CFU/ml and/or CCU/ml into units obtained during PCR analysis--geq/ml.

Materials and methods: Pure cultures of Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum were studied by cultural and molecular-biological methods with quantitative evaluation. Studies of initial cultures as well as series of 10-fold dilutions were carried out. 32 experiments in total were carried out.

Results: Ratio between geq/ml and CFU/ml for M. hominis was 3.5; geq/ ml and CCU/ml ratio--4.4. Ratio between geq/ml and CCU/ml for U. parvum was 7.1; for U. urealyticum--11.2.

Conclusion: Ratios between indexes obtained during quantitative study of pure genital micoplasma cultures by using 2 methods were established.

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