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Comparative Study
. 2014 Jul;72(7):500-5.
doi: 10.1590/0004-282x20140076.

Portable lactate analyzer for measuring lactate in cerebrospinal fluid (CSF) and plasma - method-comparison evaluations

Sérgio Monteiro de Almeida  1 , Jennifer Marquie-Beck  1 , Archana Bhatt  2 , Scott Letendre  3 , Allen McCutchan  3 , Ron Ellis  1 ; HNRC Group
Collaborators, Affiliations
Comparative Study

Portable lactate analyzer for measuring lactate in cerebrospinal fluid (CSF) and plasma - method-comparison evaluations

Sérgio Monteiro de Almeida et al. Arq Neuropsiquiatr. 2014 Jul.

Abstract

Increased plasma lactate levels can indicate the presence of metabolic disorders in HIV infected individuals.

Objective: To determine whether a portable analyzer is valid for measuring cerebrospinal fluid (CSF) and plasma lactate levels in HIV infected individuals.

Method: CSF and plasma were collected from 178 subjects. Samples tested by the Accutrend portable analyzer were compared to those tested by a reference device (SYNCHRON LX 20).

Results: The portable analyzer had in plasma sensitivity of 0.95 and specificity 0.87. For CSF the specificity was 0.95; the sensitivity 0.33; the negative predictive value was 95% and the positive predictive value 33%.

Conclusions: These findings support the validity of the portable analyzer in measuring lactate concentrations in CSF that fall within the normal range. The relatively poor positive predictive value indicates that a result above the reference range may represent a "false positive test", and should be confirmed by the reference device before concluding abnormality.

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Conflict of interest statement

Conflict of interest: There is no conflict of interest to declare.

Figures

Figure 1
Figure 1
Correlation of plasma (A) and cerebrospinal fluid (CSF) (B) lactate levels measured by the portable analyzer and reference device. The dashed horizontal and vertical lines indicate the cutoff for normal lactate levels in plasma and CSF.
Figure 2
Figure 2
Altman-Bland plots comparing lactate levels measured in plasma (A) and cerebrospinal fluid (CSF) (B) by the portable analyzer and reference device.
See this image and copyright information in PMC

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