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. 2014 Dec;118(6):661-4.
doi: 10.1016/j.jbiosc.2014.05.019. Epub 2014 Jul 22.

Differentiation of industrial sake yeast strains by a loop-mediated isothermal amplification method that targets the PHO3 gene

Takashi Kuribayashi  1 , Keigo Sato  2 , Daisuke Kasai  3 , Masao Fukuda  3 , Mitsuoki Kaneoke  1 , Ken-ichi Watanabe  1
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Differentiation of industrial sake yeast strains by a loop-mediated isothermal amplification method that targets the PHO3 gene

Takashi Kuribayashi et al. J Biosci Bioeng. 2014 Dec.

Abstract

We developed a loop-mediated isothermal amplification method that targets the PHO3 gene for discriminating sake yeast strains. Our data indicate that this assay is simple, rapid, and useful to use for differentiation of specific yeasts in sake mash.

Keywords: Acid phosphatase; Differentiation; Loop-mediated isothermal amplification; PHO3; Sake yeast.

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