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Comment
. 2014 Sep 1;33(17):1845-6.
doi: 10.15252/embj.201489450. Epub 2014 Jul 24.

Traffic control: adaptor proteins guide dynein-cargo takeoff

Michael A Cianfrocco  1 , Andres E Leschziner  2
Affiliations
Comment

Traffic control: adaptor proteins guide dynein-cargo takeoff

Michael A Cianfrocco et al. EMBO J. .

Abstract

The precise movement of intracellular components requires active transport by molecular motors along the filamentous tracks of the cytoskeleton. While yeast cytoplasmic dynein can walk for some distance along microtubules, mammalian dynein is non-processive. This has raised the question of how this motor can transport cargo. In two recent papers by the Carter, Bullock and Vale labs, mammalian dynein processivity has now been successfully reconstituted in vitro in the presence of adaptor proteins.

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Figures

Figure 1
Figure 1. BicaudalD-2 promotes the formation of a processive dynein–dynactin complex
Recombinant human dynein alone is not processive in vitro. The addition of either dynactin or BicD2N does not stimulate processivity. The combined presence of BicD2N, dynactin and dynein results in a highly processive dynein molecule. Other adaptors, such as Spindly, Rab11-FIP3, and Hook3, also increase dynein's processivity in the presence of dynactin.
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References

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