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Review
. 2014 Dec;32(12):3037-45.
doi: 10.1002/stem.1797.

Concise review: tissue-specific microvascular endothelial cells derived from human pluripotent stem cells

Hannah K Wilson  1 , Scott G Canfield, Eric V Shusta, Sean P Palecek
Affiliations
Review

Concise review: tissue-specific microvascular endothelial cells derived from human pluripotent stem cells

Hannah K Wilson et al. Stem Cells. 2014 Dec.

Abstract

Accumulating evidence suggests that endothelial cells (ECs) display significant heterogeneity across tissue types, playing an important role in tissue regeneration and homeostasis. Recent work demonstrating the derivation of tissue-specific microvascular endothelial cells (TS-MVECs) from human pluripotent stem cells (hPSCs) has ignited the potential to generate tissue-specific models which may be applied to regenerative medicine and in vitro modeling applications. Here, we review techniques by which hPSC-derived TS-MVECs have been made to date and discuss how current hPSC-EC differentiation protocols may be directed toward tissue-specific fates. We begin by discussing the nature of EC tissue specificity in vivo and review general hPSC-EC differentiation protocols generated over the last decade. Finally, we describe how specificity can be integrated into hPSC-EC protocols to generate hPSC-derived TS-MVECs in vitro, including EC and parenchymal cell coculture, directed differentiation, and direct reprogramming strategies.

Keywords: Developmental biology; Differentiation; Endothelial differentiation; Pluripotent stem cells; Tissue Specificity; Vascular development.

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Figures

Figure 1
Figure 1
In vivo and in vitro approaches to generate hPSC-derived tissue-specific microvascular endothelial cells.
See this image and copyright information in PMC

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