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. 2014 Jun;37(2):360-3.
doi: 10.1590/s1415-47572014005000010.

Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology

Ana Paula Rebelo Cassel  1 , Regina Bones Barcellos  2 , Cláudia Maria Dornelles da Silva  2 , Sabrina Esteves de Matos Almeida  3 , Maria Lucia Rosa Rossetti  4
Affiliations

Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology

Ana Paula Rebelo Cassel et al. Genet Mol Biol. 2014 Jun.

Abstract

The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HPV-DNA positive samples and in 36 out of 138 (26.1%) HPV-DNA negative ones. MN presence was significantly higher in HPV-DNA positive samples (p = 0.016). On the other hand, the absence of MN observed in a considerable number of HPV-DNA negative samples (102) may be of great value in predicting the absence of HPV. The mean age of HPV-DNA positive women (34.2 ± 12.6) was significantly lower than the mean age of HPV-DNA negative women (43.9 ± 13.7) (p = 0.003). Infection by one or multiple HPV types was found in 11 out of 20 (55.0%) and 9 out of 20 (45.0%) samples, respectively. The evaluation of MN using cervical smears collected for cytology tests could, thus, be used as additional information to monitor a population's exposure to HPV.

Keywords: Southern Brazil; cytology; high-risk HPV; human papillomavirus; micronucleus.

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References

    1. Aires GM, Meireles JR, Oliveira PC, Oliveira JL, Araújo EL, Pires BC, Cruz ES, Jesus NF, Pereira CA, Cerqueira EM. Micronuclei as biomarkers for evaluating the risk of malignant transformation in the uterine cervix. Genet Mol Res. 2011;10:1558–1564. - PubMed
    1. Barcellos RB, Almeida SE, Sperhacke RD, Verza M, Rosso F, Medeiros RM, Perizzolo PF, Cortez-Herrera E, Rossetti ML. Evaluation of a novel microplate colorimetric hybridization genotyping assay for human papillomavirus. J Virol Methods. 2011;177:38–43. - PubMed
    1. Bradford L, Goodman A. Cervical cancer screening and prevention in low-resource settings. Clin Obstet Gynecol. 2013;56:76–87. - PubMed
    1. Bruni L, Diaz M, Castellsagué X, Ferrer E, Bosch FX, de Sanjosé S. Cervical human papillomavirus prevalence in 5 continents: meta-analysis of 1 million women with normal cytological findings. J Infect Dis. 2010;202:1789–1799. - PubMed
    1. Campos LMR, Luz Dias FD, Antunes LM, Murta EF. Prevalence of micronuclei in exfoliated uterine cervical cells from patients with risk factors for cervical cancer. Sao Paulo Med J. 2008;126:323–328. - PMC - PubMed

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