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. 2014 Sep;8(5):585-6.
doi: 10.1111/irv.12261. Epub 2014 Jul 29.

Heat inactivation of the Middle East respiratory syndrome coronavirus

India Leclercq  1 , Christophe Batéjat, Ana M Burguière, Jean-Claude Manuguerra
Affiliations

Heat inactivation of the Middle East respiratory syndrome coronavirus

India Leclercq et al. Influenza Other Respir Viruses. 2014 Sep.

Abstract

The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log10 . Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.

Keywords: Heat; MERS-CoV; inactivation.

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