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. 2014 May;11(4):770-6.
doi: 10.1123/jpah.2012-0278.

Recent physical activity in relation to DNA damage and repair using the comet assay

Stephanie Whisnant Cash  1 , Shirley A Beresford, Thomas L Vaughan, Patrick J Heagerty, Leslie Bernstein, Emily White, Marian L Neuhouser
Affiliations

Recent physical activity in relation to DNA damage and repair using the comet assay

Stephanie Whisnant Cash et al. J Phys Act Health. 2014 May.

Abstract

Background: Limited evidence suggests that very high-intensity exercise is positively associated with DNA damage but moderate exercise may be associated with DNA repair.

Methods: Participants were 220 healthy, Washington State 50- to 76-year-olds in the validity/biomarker substudy of the VITamins And Lifestyle (VITAL) cohort, who provided blood samples and completed questionnaires assessing recent physical activity and demographic and health factors. Measures included nested activity subsets: total activity, moderate- plus high-intensity activity, and high-intensity activity. DNA damage (n = 122) and repair (n = 99) were measured using the comet assay. Multivariate linear regression was used to estimate regression coefficients and associated 95% confidence intervals (CIs) for relationships between MET-hours per week of activity and each DNA outcome (damage, and 15- and 60-minute repair capacities).

Results: DNA damage was not associated with any measure of activity. However, 60-minute DNA repair was positively associated with both total activity (β = 0.21, 95% CI: 0.0057-0.412; P = .044) and high-intensity activity (β = 0.31, 95% CI: 0.20-0.60; P = .036), adjusting for age, sex, BMI, and current multivitamin use.

Conclusions: This study is the first to assess broad ranges of activity intensity levels related to DNA damage and repair. Physical activity was unrelated to DNA damage but was associated with increased repair.

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Conflict of interest statement

Conflicts of Interest: None to disclose

References

    1. Ames BN. Endogenous oxidative DNA damage, aging, and cancer. Free Radic Res Commun. 1989;7(3–6):121–128. - PubMed
    1. Loft S, Poulsen HE. Cancer risk and oxidative DNA damage in man. J Mol Med. 1996 Jun;74(6):297–312. - PubMed
    1. Bernstein L, Henderson BE, Hanisch R, Sullivan-Halley J, Ross RK. Physical exercise and reduced risk of breast cancer in young women. J Natl Cancer Inst. 1994 Sep 21;86(18):1403–1408. - PubMed
    1. McTiernan A, Kooperberg C, White E, et al. Recreational physical activity and the risk of breast cancer in postmenopausal women: the Women’s Health Initiative Cohort Study. JAMA. 2003 Sep 10;290(10):1331–1336. - PubMed
    1. Dallal CM, Sullivan-Halley J, Ross RK, et al. Long-term recreational physical activity and risk of invasive and in situ breast cancer: the California teachers study. Arch Intern Med. 2007 Feb 26;167(4):408–415. - PubMed

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