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. 1978 Apr 4;508(2):364-9.
doi: 10.1016/0005-2736(78)90338-3.

NMR kinetic studies of the ionophore X-537A-mediated transport of manganous ions across phospholipid bilayers

H Degani

NMR kinetic studies of the ionophore X-537A-mediated transport of manganous ions across phospholipid bilayers

H Degani. Biochim Biophys Acta. .

Abstract

Nuclear magnetic resonance spectroscopy has been applied as a method for studying manganous ions transport across the membrane of phosphatidylcholine vesicles. The rates of the ionophore X-537A (lasalocid A)-mediated Mn2+ transport have been measured as a function of ionophore concentration, pH of the vesicle suspension, and temperature. The translocation was found to occur via a neutral complex composed of one manganous ion bound in two ionized X-537A molecules (Mn X2). The activation energy for the overall transport process was determined to be 22 +/- 5 kcal/mol. Also a pKa of 5.0 +/- 0.2 was determined for the ionophore acid dissociation equilibrium in the vesicle suspension.

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