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. 2014;19(2):184-188.
doi: 10.1007/s40502-014-0094-7. Epub 2014 Jul 16.

Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)

Devendra Kumar  1 , Dolly Wattal Dhar  1 , Sunil Pabbi  1 , Neeraj Kumar  2 , Suresh Walia  3
Affiliations

Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)

Devendra Kumar et al. Indian J Plant Physiol. 2014.

Abstract

In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A620/A280). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A620/A280) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD).

Keywords: DEAE-Cellulose-11; Phycocyanin; Spirulina.

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Figures

Fig. 1
Fig. 1
Purity of phycocyanin attained at different steps with a linear gradient of pH from 3.76 to 5.1
Fig. 2
Fig. 2
Absorbtion spectrum of purified Phycocyanin
Fig. 3
Fig. 3
Revere phase-HPLC profile of phycocyanin from Spirulina strain using PDA Detector (620 nm) showing α and β subunit peaks
Fig. 4
Fig. 4
a Absorbtion spectra of α-subunit with RT 25.612. b Absorpsion spectra of β-subunit with RT 27.024
Fig. 5
Fig. 5
SDS-PAGE of purified phycocyanin (M molecular marker, lane 1 PC)
See this image and copyright information in PMC

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