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. 2014:2014:141675.
doi: 10.1155/2014/141675. Epub 2014 Jun 30.

Molecular cloning and bioinformatics analysis of a new plasma membrane Na⁺/H⁺ antiporter gene from the halophyte Kosteletzkya virginica

Hongyan Wang  1 , Xiaoli Tang  2 , Chuyang Shao  3 , Hongbo Shao  4 , Honglei Wang  5
Affiliations

Molecular cloning and bioinformatics analysis of a new plasma membrane Na⁺/H⁺ antiporter gene from the halophyte Kosteletzkya virginica

Hongyan Wang et al. ScientificWorldJournal. 2014.

Abstract

A new plasma membrane Na(+)/H(+) antiporter gene (named as KvSOS1) was cloned from the halophyte Kosteletzkya virginica by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology, which is a homologue of SOS1 (salt overly sensitive 1). The full-length cDNA is 3850 bp and contains an open reading frame (ORF) encoding a protein of 1147 amino acids with a molecular weight of 127.56 kDa and a theoretical pI of 6.18. Bioinformatics analysis indicated that the deduced protein appears to be a transmembrane protein with 12 transmembrane domains at the N-terminal region and a long hydrophilic tail in cytoplasm at its C-terminal region and shares 72-82% identity at the peptide level with other plant plasma membrane Na(+)/H(+) antiporters.

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Figures

Figure 1
Figure 1
Analysis of conserved domains in KvSOS1.
Figure 2
Figure 2
Plot of hydrophobic and hydrophilic areas in KvSOS1.
Figure 3
Figure 3
Alignment of KvSOS1 with other SOS1 homologues derived from Theobroma cacao (TcSOS1, EOY01238.1), Populus trichocarpa (PtSOS1, XP_002315837.2), Ricinus communis (RcSOS1, XP_002521897.1), Bruguiera gymnorrhiza (BgSOS1, ADK91080.1), Cucumis sativus (CsSOS1, XP_004150155.1), Vitis vinifera (VvSOS1, NP_001268140.1), and Zygophyllum xanthoxylum (ZxSOS1, ACZ57357.1). Identical peptides highlighted in black.
Figure 4
Figure 4
Phylogeny of KvSOS1 and other Na+/H+antiporter proteins derived from Populus trichocarpa (PtSOS1, XP_002315837.2), Populus euphratica (PeSOS1, ABF60872.1), Ricinus communis (RcSOS1, XP_002521897.1), Theobroma cacao (TcSOS1, EOY01238.1), Citrus sinensis (CsSOS1, XP_006492282.1), Glycine max (GmSOS1, AFD64746.1), Lolium perenne (LpSOS1, AAY42598.1), Solanum lycopersicum (SlSOS1, NP_001234698.1), Limonium gmelinii (LgSOS1, ACF05808.1), Arabidopsis thaliana (AtSOS1, AF256224_1), Theobroma cacao (TcNHX1, XP_007030791.1), Populus euphratica (PeNHX1, ACZ05630.1), Solanum torvum (StNHX1, AEN04067.1), Triticum aestivum (TaNHX1, AAS17949.1), Arabidopsis thaliana (AtNHX1, NP_198067.1), Halostachys caspica (HcNHX1, ADK62565.1), Zoysia japonica (ZjNHX1, ABY19311.2), and Aeluropus littoralis (AlNHX1, AAV80466.1).
Figure 5
Figure 5
The ORF nucleotide sequence and the deduced peptide sequence of KvSOS1. Start codon and termination codon are highlighted in red.
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