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Review
. 1989:52 Suppl:55-62.
doi: 10.1679/aohc.52.suppl_55.

In situ hybridization using biotin-labeled oligonucleotides: probe labeling and procedures for mRNA detection

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Review

In situ hybridization using biotin-labeled oligonucleotides: probe labeling and procedures for mRNA detection

L I Larsson. Arch Histol Cytol. 1989.

Abstract

Methods for non-radioactive in situ hybridization are rapidly progressing in refinement and application. In this overview, we present our experiences with the use of synthetic oligodeoxynucleotides, labeled with biotin- or digoxigenin-tagged nucleotides by the terminal transferase method.

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