In situ hybridization using biotin-labeled oligonucleotides: probe labeling and procedures for mRNA detection
- PMID: 2510803
- DOI: 10.1679/aohc.52.suppl_55
In situ hybridization using biotin-labeled oligonucleotides: probe labeling and procedures for mRNA detection
Abstract
Methods for non-radioactive in situ hybridization are rapidly progressing in refinement and application. In this overview, we present our experiences with the use of synthetic oligodeoxynucleotides, labeled with biotin- or digoxigenin-tagged nucleotides by the terminal transferase method.
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