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. 1989;7(3-6):293-300.
doi: 10.3109/10715768909087954.

31P-NMR spectroscopy of phosphate compartmentation during ischaemia in hearts protected by cardioplegic treatment

A Stier  1 , S A Finch, H Schäfer, M M Gebhardt, H J Bretschneider
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31P-NMR spectroscopy of phosphate compartmentation during ischaemia in hearts protected by cardioplegic treatment

A Stier et al. Free Radic Res Commun. 1989.

Abstract

Four tissue compartments, differing in proton and inorganic phosphate concentration, were resolved by 31P-NMR spectroscopy in samples from dog hearts after cardioplegic treatment with HTK solution. Inversion of the physiological cytoplasmic-mitochondrial pH gradient was observed. The considerable ensuing acidosis of the matrix is discussed with regard to a possible delocalization of ferrous ions.

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