Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Aug;80(8):754-8.
doi: 10.1111/j.1349-7006.1989.tb01710.x.

Similarity in the effect of caffeine on DNA synthesis after UV irradiation between xeroderma pigmentosum variant cells and mouse cells

T Yagi  1 , H Takebe
Affiliations

Similarity in the effect of caffeine on DNA synthesis after UV irradiation between xeroderma pigmentosum variant cells and mouse cells

T Yagi et al. Jpn J Cancer Res. 1989 Aug.

Abstract

The effect of UV irradiation on the rate of DNA synthesis was compared among normal human, xeroderma pigmentosum (XP, group A and variant) and mouse cells with and without caffeine in the culture medium after UV irradiation. At the same levels of survival, approximately 37%, all cells showed reduction in the rate of synthesis 0-3 h after UV irradiation followed by a recovery to normal or near-normal level 12 h later. In the presence of caffeine, no change in the recovery patterns was observed in normal human and XP A cells. XP variant cells and mouse cells showed little or no recovery in the presence of caffeine even after 12 h, when full recovery was obtained without caffeine. XP variant and mouse cells appear to have a common response in that post-irradiation treatment with caffeine inhibits reinitiation of UV-reduced DNA replication. Enhancement by caffeine of UV-killing in XP variant and mouse cells may be due to the retarded resumption of DNA replication.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. ) Cleaver , J. E.Investigations into the effect of ultraviolet light on the rate of deoxyribonucleic acid synthesis in mammalian cells . Biochim. Biophys. Acta , 108 , 42 – 52 ( 1965. ). - PubMed
    1. ) Domon , M. and Rauth , A. M.Ultraviolet irradiation of mouse L cells: effects on DNA synthesis and progression through the cell cycle . Radial Res. , 35 , 350 – 368 ( 1968. ). - PubMed
    1. ) Rude , J. M , and Friedberg , E. C.Semi‐conservative deoxyribonucleic acid synthesis in unirradiated and ultraviolet‐irradiated xeroderma pigmentosum and normal human skin fibroblasts . Mutat. Res. , 42 , 433 – 442 ( 1977. ). - PubMed
    1. ) Moustacchi , E. , Ehmann , U. K. and Friedberg , E. C.Defective recovery of semi‐conservative DNA synthesis in xeroderma pigmentosum cells following split‐dose ultraviolet irradiation . Mutat. Res. , 62 , 159 – 171 ( 1979. ). - PubMed
    1. ) Ikenaga , M. , Inoue , M. , Kozuka , T. and Sugita , T.The recovery of colony‐forming ability and the rate of semi‐conservative DNA synthesis in ultraviolet‐irradiated Cockayne and normal human cells . Mutat. Res. , 91 , 87 – 91 ( 1981. ). - PubMed

Publication types

LinkOut - more resources