Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Aug 12:13:42.
doi: 10.1186/s12941-014-0042-4.

Spread of carbapenemase-producing enterobacteria in a southwest hospital in China

Sheng Chen, Wei Feng, Jianhong Chen, Wei Liao, Nianhai He, Qian Wang, Fengjun Sun  1 , Peiyuan Xia
Affiliations

Spread of carbapenemase-producing enterobacteria in a southwest hospital in China

Sheng Chen et al. Ann Clin Microbiol Antimicrob. .

Abstract

Background: The rapid emergence and dissemination of carbapenem resistance in Enterobacteriaceae complicates the treatment of infections caused by these organisms.

Methods: We collected clinical isolates with meropenem inhibition zones of ≤ 22 mm from January 1, 2009, through December 31, 2010. We attempted to amplify the NDM-1 gene from these isolates and conducted the modified Hodge test (MHT). The minimal inhibitory concentration (MIC) of the MHT-positive strains was determined by the agar disk dilution method. The carbapenemase-encoding resistance genes of these strains were examined using polymerase chain reaction (PCR) analysis and a sequencing strategy to characterize these enzymes. The clonal relationship among isolates was analyzed by pulsed-field gel electrophoresis (PFGE).

Results: Among the 158 Enterobacteriaceae isolates that were collected, there were no NDM-1-positive strains and 26 MHT-positive strains. Among the latter, 18 strains were IMP-4-positive, and 1 was KPC-2-positive. In addition, 15 of the IMP-4-positive Klebsiella pneumoniae strains belonged to 4 PFGE genotypes, with 8 strains having the same genotype.

Conclusion: These results suggest that nosocomial infections are one of the main reasons for the spread of these resistant strains.

PubMed Disclaimer

Figures

Figure 1
Figure 1
PFGE analysis of genomic DNA from isolates of K. pneumoniae.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Logan LK. Carbapenem-resistant enterobacteriaceae: an emerging problem in children. Clin Infect Dis. 2012;55:852–859. doi: 10.1093/cid/cis543. - DOI - PubMed
    1. Nordmann P, Naas T, Poirel L. Global spread of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis. 2011;17:1791–1798. doi: 10.3201/eid1710.110655. - DOI - PMC - PubMed
    1. Voulgari E, Poulou A, Koumaki V, Tsakris A. Carbapenemase-producing Enterobacteriaceae: now that the storm is finally here, how will timely detection help us fight back? Future Microbiol. 2013;8:27–39. doi: 10.2217/fmb.12.130. - DOI - PubMed
    1. Thurlow CJ, Prabaker K, Lin MY, Lolans K, Weinstein RA, Hayden MK. Anatomic sites of patient colonization and environmental contamination with klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae at long-term acute care hospitals. Infect Control Hosp Epidemiol. 2013;34:56–61. doi: 10.1086/668783. - DOI - PMC - PubMed
    1. Lee K, Chong Y, Shin HB, Kim YA, Yong D, Yum JH. Modified Hodge and EDTA-disk synergy tests to screen metallo-β-lactamase-producing strains of pseudomonas and acinetobacter species. Clin Microbiol Infect. 2001;7:88–91. doi: 10.1046/j.1469-0691.2001.00204.x. - DOI - PubMed

MeSH terms

LinkOut - more resources