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. 2014 Jun 15;7(7):3724-32.
eCollection 2014.

Co-expression of delta-catenin and RhoA is significantly associated with a malignant lung cancer phenotype

Di Zhang  1 , Jun-Yi Zhang  2 , Shun-Dong Dai  1 , Shu-Li Liu  1 , Yang Liu  1 , Na Tang  1 , En-Hua Wang  1
Affiliations

Co-expression of delta-catenin and RhoA is significantly associated with a malignant lung cancer phenotype

Di Zhang et al. Int J Clin Exp Pathol. .

Abstract

Delta-catenin, a member of the p120-catenin subfamily, and the Rho GTPase RhoA both have roles in the regulation of the cytoskeleton. In this study, we found that delta-catenin positive expression and RhoA over-expression is consistently found in non-small cell lung cancer, but not in normal lung tissue, and that their co-expression was significantly associated with histological type, differentiation, pTNM stage, lymphatic metastasis and a poor prognosis. We also demonstrate that delta-catenin can directly interact with RhoA and regulate its activity, which in turn mediates tumor invasion and metastasis.

Keywords: GTPase; RhoA; co-expression; delta-catenin; non-small cell lung cancer; prognosis.

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Figures

Figure 1
Figure 1
Delta-catenin and RhoA expression in normal bronchial epithelium, lung squamous cell carcinoma and adenocarcinoma. Delta-catenin (A) and RhoA (B) was weakly expressed in the cytoplasm of normal bronchial epithelialcells.Positive delta-catenin expression was detected in the cytoplasm of squamous cell carcinoma (C), and RhoA was strongly expressed in these cells (D). Delta-catenin was expressed (E) and RhoA (F) was over-expressed in adenocarcinoma.
Figure 2
Figure 2
Co-expression of delta-catenin and RhoA is associated with a poor prognosis. The survival time of patients with both delta-catenin and RhoA tumor expression was shorter than those with either delta-catenin expression or RhoA over-expression alone.
Figure 3
Figure 3
Delta-catenin and RhoA are expressed to a significantly higher level in lung cancer compared to normal lung tissue. Delta-catenin and RhoA protein expression in lung cancer (T1-T3) was significantly higher than in adjacent normal lung tissue (N1-N3) (A, B). Delta-catenin and RhoA mRNA levels in lung cancer (T1-T3) were also significantly higher than in adjacent normal lung tissue (N1-N3) (C, D).
Figure 4
Figure 4
Interaction of delta-catenin and RhoA in lung cancer cells. The sub-cellular location of delta-catenin (A) and RhoA (B) in H460 cells, and their co-localization (C). The sub-cellular location of delta-catenin (D) and RhoA (E) in LK2 cells, and their co-localization (F). Co-immunoprecipitation of delta-catenin and RhoA in H460 and LK2 cells (G, H).
Figure 5
Figure 5
RhoA and delta-catenin expression. RhoA expression was reduced after delta-catenin expression (A, B), and elevated after the forced expression of delta-catenin (C, D). Elevated delta-catenin expression reduced RhoA activity in LK2 cells (E), and RhoA activity increased after silencing delta-catenin in H460 cells (F). *P<0.05.
Figure 6
Figure 6
Reduction of delta-catenin in H460 cells inhibited cell invasion, and the over-expression of delta-catenin in LK2 cells promoted cancer cell invasion. *P<0.05.
See this image and copyright information in PMC

References

    1. Hatzfeld M. The p120 family of cell adhesion molecules. Eur J Cell Biol. 2005;84:205–14. - PubMed
    1. Kosik KS, Donahue CP, Israely I, Liu X, Ochiishi T. delta-catenin at the synaptic-adherens junction. Trends Cell Biol. 2005;15:172–178. - PubMed
    1. Kawamura Y, Fan QW, Hayashi H, Michikawa M, Yanagisawa K, Komano H. Expression of the mRNA for two isoforms of neural plakophilin- related arm-repeat protein/delta-catenin in rodent neurons and glial cells. Neurosci Lett. 1999;277:185–8. - PubMed
    1. Lu Q, Paredes M, Medina M, Zhou J, Cavallo R, Peifer M, Orecchio L, Kosik KS. delta-catenin, an adhesive junction associated protein which promotes cell scattering. J Cell Biol. 1999;144:519–532. - PMC - PubMed
    1. Koutras C, Lessard CB, Lévesque G. A nuclear function for the presenilin 1 neuronal partner NPRAP/δ-catenin. J Alzheimers Dis. 2011;27:307–16. - PubMed

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