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. 2014 Aug;93(8):e51.
doi: 10.1097/MD.0000000000000051.

Characteristics of fecal and mucosa-associated microbiota in Chinese patients with inflammatory bowel disease

Liping Chen  1 , Wei Wang, Rui Zhou, Siew C Ng, Jin Li, Meifang Huang, Feng Zhou, Xin Wang, Bo Shen, Michael A Kamm, Kaichun Wu, Bing Xia
Affiliations

Characteristics of fecal and mucosa-associated microbiota in Chinese patients with inflammatory bowel disease

Liping Chen et al. Medicine (Baltimore). 2014 Aug.

Abstract

The intestinal microbiota plays an important role in the pathogenesis of inflammatory bowel disease (IBD), and geographical and genetic backgrounds impact the composition of the intestinal microbiota. However, there is a lack of evidence regarding the overall changes and characteristics of fecal-associated microbiota (FAM) and mucosa-associated microbiota (MAM) in Chinese patients with IBD. We recruited 26 patients with Crohn's disease (CD), 46 patients with ulcerative colitis (UC), and 21 healthy individuals; we collected matched fresh fecal and mucosal samples from the same subjects. The microbial communities were studied by 454-pyrosequencing. Community-wide changes in FAM and MAM were observed in patients with IBD. The proportion of several butyrate-producing bacteria, such as of the genera Roseburia, Coprococcus, and Ruminococcus were significantly reduced, whereas the pathogens Escherichia-Shigella and Enterococcus were prevalent in patients with IBD. FAM and MAM were similar between CD and UC. FAM differed from MAM in healthy individuals and patients with UC. In conclusion, the compositions of FAM and MAM were altered in patients with IBD. The reduction of butyrate-producing bacteria and the increase in opportunistic pathogens might be associated with the pathogenesis of IBD.

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Conflict of interest statement

The authors have no conflicts of interest to disclose.

Figures

FIGURE 1
FIGURE 1
Relative abundance of primary bacterial phyla in different groups of samples. “Others” represents the Synergistetes, TM7, Tenericutes, Verrucomicrobia, Lentisphaerae, Acidobacteria, Gemmatimonadetes, Nitrospira, Planctomycetes, SR1, Spirochaetes, Armatimonadetes, Chloroflexi, and OD1. The first 5 phyla were not apparent in stool samples. “Unknown” represents the unclassified bacteria.
FIGURE 2
FIGURE 2
PCA plots based on unweighted Unifrac metrics. Each symbol represents a sample. (A) Groups HF, CF, and UF. (B) Groups HI and CI. (C) Groups HC, CC, and UC. (D) Groups HR, CR, and UR. (E) Groups HF, HI, HC, and HR. (F) Groups HI, HC, and HR. (G) Groups UF, UC, and UR. (H) Groups UC and UR.
FIGURE 3
FIGURE 3
Unweighted Unifrac distance between matched feces and various mucosal samples from the same subject. (A) Unweighted Unifrac distance of healthy individuals. ∗P < 0.001, compared with the feces/ileum group; ψP < 0.001, compared with the feces/cecum group; τP < 0.001, compared with the feces/rectum group. (B) Unweighted Unifrac distance of UC patients. ∗P < 0.001, compared with the feces/cecum group; ψP < 0.001, compared with the feces/rectum group.
See this image and copyright information in PMC

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